Article (Scientific journals)
The sequential activity of the GTPases Rap1B and Cdc42 determines neuronal polarity.
SCHWAMBORN, Jens Christian; Puschel, Andreas W.
2004In Nature Neuroscience, 7 (9), p. 923-9
Peer Reviewed verified by ORBi
 

Files


Full Text
2004_NatNeuro.pdf
Publisher postprint (1.08 MB)
Request a copy

All documents in ORBilu are protected by a user license.

Send to



Details



Keywords :
Animals; Animals, Newborn; Antibodies, Monoclonal/metabolism; Axons/drug effects/metabolism; Body Patterning/drug effects/physiology; Carrier Proteins/genetics/metabolism; Cells, Cultured; Cytochalasin D/pharmacology; Dendrites/drug effects/metabolism; Embryo, Mammalian; GTP Phosphohydrolases/physiology; Gene Transfer Techniques; Green Fluorescent Proteins; Hippocampus/cytology; Immunohistochemistry/methods; Luminescent Proteins/metabolism; Mice; Microtubule-Associated Proteins/metabolism; Neurons/cytology/drug effects/physiology; Phalloidine/drug effects; Protein Kinase C/metabolism; Protein-Serine-Threonine Kinases/metabolism; Proto-Oncogene Proteins/metabolism; Proto-Oncogene Proteins c-akt; RNA Interference/physiology; RNA, Messenger/biosynthesis; Rats; Reverse Transcriptase Polymerase Chain Reaction/methods; Time Factors; Transfection/methods; cdc42 GTP-Binding Protein/physiology; rap GTP-Binding Proteins/physiology; rhoA GTP-Binding Protein/genetics/metabolism
Abstract :
[en] The establishment of a polarized morphology is an essential step in the differentiation of neurons with a single axon and multiple dendrites. In cultured rat hippocampal neurons, one of several initially indistinguishable neurites is selected to become the axon. Both phosphatidylinositol 3,4,5-trisphosphate and the evolutionarily conserved Par complex (comprising Par3, Par6 and an atypical PKC (aPKC) such as PKClambda or PKCzeta) are involved in axon specification. However, the initial signals that establish cellular asymmetry and the pathways that subsequently translate it into structural changes remain to be elucidated. Here we show that localization of the GTPase Rap1B to the tip of a single neurite is a decisive step in determining which neurite becomes the axon. Using GTPase mutants and RNA interference, we found that Rap1B is necessary and sufficient to initiate the development of axons upstream of Cdc42 and the Par complex.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
SCHWAMBORN, Jens Christian ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Puschel, Andreas W.
Language :
English
Title :
The sequential activity of the GTPases Rap1B and Cdc42 determines neuronal polarity.
Publication date :
2004
Journal title :
Nature Neuroscience
ISSN :
1546-1726
Publisher :
Nature Publishing Group, United Kingdom
Volume :
7
Issue :
9
Pages :
923-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 21 June 2013

Statistics


Number of views
124 (2 by Unilu)
Number of downloads
0 (0 by Unilu)

Scopus citations®
 
334
Scopus citations®
without self-citations
314
OpenCitations
 
306
WoS citations
 
317

Bibliography


Similar publications



Contact ORBilu