Reference : Direct reprogramming of fibroblasts into neural stem cells by defined factors.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/2730
Direct reprogramming of fibroblasts into neural stem cells by defined factors.
English
Han, Dong Wook [> >]
Tapia, Natalia [> >]
Hermann, Andreas [> >]
Hemmer, Kathrin [> >]
Hoing, Susanne [> >]
Arauzo-Bravo, Marcos J. [> >]
Zaehres, Holm [> >]
Wu, Guangming [> >]
Frank, Stefan [> >]
Moritz, Soren [> >]
Greber, Boris [> >]
Yang, Ji Hun [> >]
Lee, Hoon Taek [> >]
Schwamborn, Jens Christian mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Storch, Alexander [> >]
Scholer, Hans R. [> >]
2012
Cell Stem Cell
10
4
465-72
Yes (verified by ORBilu)
International
1875-9777
United States
[en] Animals ; Antigens, Differentiation/biosynthesis/genetics ; Cell Dedifferentiation ; Fibroblasts/cytology/metabolism ; Gene Expression Regulation/genetics ; Induced Pluripotent Stem Cells/cytology/metabolism ; Mice ; Neural Stem Cells/cytology/metabolism ; Transcription Factors/biosynthesis/genetics
[en] Recent studies have shown that defined sets of transcription factors can directly reprogram differentiated somatic cells to a different differentiated cell type without passing through a pluripotent state, but the restricted proliferative and lineage potential of the resulting cells limits the scope of their potential applications. Here we show that a combination of transcription factors (Brn4/Pou3f4, Sox2, Klf4, c-Myc, plus E47/Tcf3) induces mouse fibroblasts to directly acquire a neural stem cell identity-which we term as induced neural stem cells (iNSCs). Direct reprogramming of fibroblasts into iNSCs is a gradual process in which the donor transcriptional program is silenced over time. iNSCs exhibit cell morphology, gene expression, epigenetic features, differentiation potential, and self-renewing capacity, as well as in vitro and in vivo functionality similar to those of wild-type NSCs. We conclude that differentiated cells can be reprogrammed directly into specific somatic stem cell types by defined sets of specific transcription factors.
http://hdl.handle.net/10993/2730
Copyright (c) 2012 Elsevier Inc. All rights reserved.

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