Article (Scientific journals)
Unidirectional P-body transport during the yeast cell cycle.
Garmendia-Torres, Cecilia; Skupin, Alexander; Michael, Sean A. et al.
2014In PLoS ONE, 9 (6), p. 99428
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Abstract :
[en] P-bodies belong to a large family of RNA granules that are associated with post-transcriptional gene regulation, conserved from yeast to mammals, and influence biological processes ranging from germ cell development to neuronal plasticity. RNA granules can also transport RNAs to specific locations. Germ granules transport maternal RNAs to the embryo, and neuronal granules transport RNAs long distances to the synaptic dendrites. Here we combine microfluidic-based fluorescent microscopy of single cells and automated image analysis to follow p-body dynamics during cell division in yeast. Our results demonstrate that these highly dynamic granules undergo a unidirectional transport from the mother to the daughter cell during mitosis as well as a constrained "hovering" near the bud site half an hour before the bud is observable. Both behaviors are dependent on the Myo4p/She2p RNA transport machinery. Furthermore, single cell analysis of cell size suggests that PBs play an important role in daughter cell growth under nutrient limiting conditions.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Integrative Cell Signalling (Skupin Group)
Luxembourg Centre for Systems Biomedicine (LCSB): Experimental Neurobiology (Balling Group)
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Garmendia-Torres, Cecilia
Skupin, Alexander  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Michael, Sean A.
Ruusuvuori, Pekka
Kuwada, Nathan J.
Falconnet, Didier
Cary, Gregory A.
Hansen, Carl
Wiggins, Paul A.
Dudley, Aimee M.
External co-authors :
yes
Language :
English
Title :
Unidirectional P-body transport during the yeast cell cycle.
Publication date :
2014
Journal title :
PLoS ONE
ISSN :
1932-6203
Publisher :
Public Library of Science, United States - California
Volume :
9
Issue :
6
Pages :
e99428
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 03 November 2015

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