Reference : Non-targeted tracer fate detection

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology Physical, chemical, mathematical & earth Sciences : Chemistry
	To cite this reference:	http://hdl.handle.net/10993/20835

Title :	Non-targeted tracer fate detection
Language :	English
Author, co-author :	Weindl, Daniel [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Wegner, André [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Hiller, Karsten [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Publication date :	2015
Journal title :	Methods in Enzymology
Volume :	561
Special issue title :	Metabolic analysis using stable isotopes
Pages :	227-302
Peer reviewed :	Yes (verified by ORBilu)
Audience :	International
ISSN :	0076-6879
Keywords :	[en] stable isotope labeling ; non-targeted ; 13C
Abstract :	[en] Stable isotopes have been used to trace atoms through metabolism and quantify metabolic fluxes for several decades. Only recently non-targeted stable isotope labeling approaches have emerged as a powerful tool to gain biological insights into metabolism. However, the manual detection of isotopic enrichment for a non-targeted analysis is tedious and time consuming. To overcome this limitation, the non-targeted tracer fate detection (NTFD) algorithm for the automated metabolome-wide detection of isotopic enrichment has been developed. NTFD detects and quantifies isotopic enrichment in the form of mass isotopomer distributions (MIDs) in an automated manner, providing the means to trace functional groups, determine MIDs for metabolic flux analysis, or detect tracer-derived molecules in general. Here, we describe the algorithmic background of NTFD, discuss practical considerations for the freely available NTFD software package, and present potential applications of non-targeted stable isotope labeling analysis.
Research centres :	Luxembourg Centre for Systems Biomedicine (LCSB): Metabolomics (Hiller Group)
Funders :	Fonds National de la Recherche - FnR
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/10993/20835
DOI :	10.1016/bs.mie.2015.04.003
Other URL :	http://www.sciencedirect.com/science/article/pii/S0076687915002645

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