Reference : Non-targeted tracer fate detection
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
Non-targeted tracer fate detection
Weindl, Daniel mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Wegner, André mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Hiller, Karsten mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Methods in Enzymology
Metabolic analysis using stable isotopes
Yes (verified by ORBilu)
[en] stable isotope labeling ; non-targeted ; 13C
[en] Stable isotopes have been used to trace atoms through metabolism and quantify metabolic fluxes for several decades. Only recently non-targeted stable isotope labeling approaches have emerged as a powerful tool to gain biological insights into metabolism. However, the manual detection of isotopic enrichment for a non-targeted analysis is tedious and time consuming. To overcome this limitation, the non-targeted tracer fate detection (NTFD) algorithm for the automated metabolome-wide detection of isotopic enrichment has been developed. NTFD detects and quantifies isotopic enrichment in the form of mass isotopomer distributions (MIDs) in an automated manner, providing the means to trace functional groups, determine MIDs for metabolic flux analysis, or detect tracer-derived molecules in general. Here, we describe the
algorithmic background of NTFD, discuss practical considerations for the freely available NTFD software package, and present potential applications of non-targeted stable isotope labeling analysis.
Luxembourg Centre for Systems Biomedicine (LCSB): Metabolomics (Hiller Group)
Fonds National de la Recherche - FnR
Researchers ; Professionals ; Students

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