Reference : Characterization of new proteins found by analysis of short open reading frames from ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Characterization of new proteins found by analysis of short open reading frames from the full yeast genome
Andrade, M. A. [> >]
Daruvar, A. [> >]
Casari, G. [> >]
Schneider, Reinhard mailto [embl]
Termier, M. [> >]
Sander, C. [> >]
Journal of Yeast and Fungal Research
Academic Journals
Yes (verified by ORBilu)
[en] Saccharomyces cerevisiae ; short ORFs ; computational ORF verification ; ORF properties ; sequence similarity
[en] We have analysed short open reading frames (between 150 and 300 base pairs long) of the yeast genome (Saccharomyces cerevisiae) with a two-step strategy. The first step selects a candidate set of open reading frames from the DNA. sequence based on statistical evaluation of DNA and protein sequence properties. The second step filters the candidate set by selecting open reading frames with high similarity to other known sequences (from any organism). As a result, we report ten new predicted proteins not present in the current sequence databases. These include a new alcohol dehydrogenase, a protein probably related to the cell cycle, as well as a homolog of the prokaryotic ribosomal protein L36 likely to be a mitochondrial ribosomal protein coded in the nuclear genome. We conclude that the analysis of short open reading frames leads to biologically interesting discoveries, even though the quantitative yield of new proteins is relatively low.

File(s) associated to this reference

Fulltext file(s):

Open access
0c96052a02d07370e3000000.pdfPublisher postprint962.67 kBView/Open

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.