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Poster (Scientific congresses, symposiums and conference proceedings)
A biomolecular isolation protocol for Eco-Systems Biology: How to isolate DNA, RNA, proteins and metabolites from a single unique sample
Roume, Hugo; Muller, Emilie; Shah, Pranjul et al.
201211th Annual International Symposium Addresses Systems Biology and the Microbiome
 

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Abstract :
[en] The Molecular Systems Biology approach based on the integration of omic datasets is hampered by the complexity, dynamic and heterogeneity of mixed microbial communities. In order to facilitate meaningful data integration, individual biomolecular fractions need to be obtained from single unique samples. Our newly developed methodology allows for the isolation of high-quality genomic DNA, RNA, proteins and metabolites from single mixed microbial community samples (e.g. human faeces, freshwater filtrate), as well as from mammalian tissues. The framework lays the basis for standardized molecular (eco-)systematic studies on a range of different biological samples in the future.
Disciplines :
Environmental sciences & ecology
Microbiology
Author, co-author :
Roume, Hugo ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Muller, Emilie ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Shah, Pranjul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Wilmes, Paul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Language :
English
Title :
A biomolecular isolation protocol for Eco-Systems Biology: How to isolate DNA, RNA, proteins and metabolites from a single unique sample
Publication date :
2012
Event name :
11th Annual International Symposium Addresses Systems Biology and the Microbiome
Event date :
from the 15-04-2012 to the 16-04-2012
Audience :
International
Available on ORBilu :
since 08 January 2015

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