Reference : The sequential isolation of metabolites, RNA, DNA, and proteins from a single, undivi... |
Scientific journals : Other | |||
Life sciences : Environmental sciences & ecology Life sciences : Microbiology | |||
http://hdl.handle.net/10993/18994 | |||
The sequential isolation of metabolites, RNA, DNA, and proteins from a single, undivided mixed microbial community sample | |
English | |
Muller, Emilie ![]() | |
Buschart, Anna ![]() | |
Roume, Hugo [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >] | |
Lebrun, Laura ![]() | |
Wilmes, Paul ![]() | |
2014 | |
Methods in Enzymology | |
Elsevier | |
No | |
International | |
[en] Biomolecular isolation ; Eco-Systems Biology ; Integrated omics ; Microbial community ; Multi-omics | |
[en] Integrated omics of microbial consortia, comprising systematized metagenomic, metatranscriptomic, metaproteomic and meta-metabolomic analyses, allows in-depth characterization of organismal and functional diversity in situ. To allow meaningful meta-omic data integration, truly systematic measurements of the typically heterogeneous sample biomass is required. Therefore, there is a need for analyzing biomolecular fractions obtained from single, undivided samples. Here, we share a methodological workflow for the reproducible isolation of concomitant polar and non-polar metabolites, RNA, DNA and proteins from samples obtained from a biological wastewater treatment plant. The methodological framework is applicable to other biological samples [1,2], is compatible with different kits for biomacromolecular isolation [1,2] with minimal tailoring, and represents an important first step in standardization for the emerging field of Molecular Eco-Systems Biology. | |
Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group) | |
http://hdl.handle.net/10993/18994 | |
10.1016/B978-0-12-407863-5.00011-3 |
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