Other (Scientific journals)The sequential isolation of metabolites, RNA, DNA, and proteins from a single, undivided mixed microbial community sample
MULLER, Emilie; BUSCHART, Anna; ROUME, Hugo et al.
2014 • In Methods in Enzymology
No document available.
Abstract :
[en] Integrated omics of microbial consortia, comprising systematized metagenomic, metatranscriptomic, metaproteomic and meta-metabolomic analyses, allows in-depth characterization of organismal and functional diversity in situ. To allow meaningful meta-omic data integration, truly systematic measurements of the typically heterogeneous sample biomass is required. Therefore, there is a need for analyzing biomolecular fractions obtained from single, undivided samples. Here, we share a methodological workflow for the reproducible isolation of concomitant polar and non-polar metabolites, RNA, DNA and proteins from samples obtained from a biological wastewater treatment plant. The methodological framework is applicable to other biological samples [1,2], is compatible with different kits for biomacromolecular isolation [1,2] with minimal tailoring, and represents an important first step in standardization for the emerging field of Molecular Eco-Systems Biology.
Title :
The sequential isolation of metabolites, RNA, DNA, and proteins from a single, undivided mixed microbial community sample
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