Reference : The sequential isolation of metabolites, RNA, DNA, and proteins from a single, undivi...

	Document type :	Scientific journals : Other
	Discipline(s) :	Life sciences : Environmental sciences & ecology Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/10993/18994

Title :	The sequential isolation of metabolites, RNA, DNA, and proteins from a single, undivided mixed microbial community sample
Language :	English
Author, co-author :	Muller, Emilie [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Buschart, Anna [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Roume, Hugo [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Lebrun, Laura [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Wilmes, Paul [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Publication date :	2014
Journal title :	Methods in Enzymology
Publisher :	Elsevier
Peer reviewed :	No
Audience :	International
Keywords :	[en] Biomolecular isolation ; Eco-Systems Biology ; Integrated omics ; Microbial community ; Multi-omics
Abstract :	[en] Integrated omics of microbial consortia, comprising systematized metagenomic, metatranscriptomic, metaproteomic and meta-metabolomic analyses, allows in-depth characterization of organismal and functional diversity in situ. To allow meaningful meta-omic data integration, truly systematic measurements of the typically heterogeneous sample biomass is required. Therefore, there is a need for analyzing biomolecular fractions obtained from single, undivided samples. Here, we share a methodological workflow for the reproducible isolation of concomitant polar and non-polar metabolites, RNA, DNA and proteins from samples obtained from a biological wastewater treatment plant. The methodological framework is applicable to other biological samples [1,2], is compatible with different kits for biomacromolecular isolation [1,2] with minimal tailoring, and represents an important first step in standardization for the emerging field of Molecular Eco-Systems Biology.
Research centres :	Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group)
Permalink :	http://hdl.handle.net/10993/18994
DOI :	10.1016/B978-0-12-407863-5.00011-3

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