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Polymorphisms of the alpha-synuclein promoter: expression analyses and association studies in Parkinson's disease.
Holzmann, C.; Krüger, Rejko; Saecker, A. M. M. Vieira et al.
2003In Journal of Neural Transmission, 110 (1), p. 67-76
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Keywords :
Aged; Cell Culture Techniques; Cysteine/genetics; Enzyme-Linked Immunosorbent Assay; Female; Gene Expression Regulation; Genes, Reporter; Glycine/genetics; Haplotypes; Humans; Male; Middle Aged; Nerve Tissue Proteins/genetics; Neuroblastoma; Parkinson Disease/genetics; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Single-Stranded Conformational; Promoter Regions, Genetic/genetics; Synucleins; Threonine/genetics; Transfection; alpha-Synuclein
Abstract :
[en] Mutations of the alpha-synuclein gene have shown to be relevant in some rare families with autosomal dominant Parkinson's disease (PD). Furthermore, alpha-synuclein protein is a major component of the Lewy bodies also in sporadic PD patients. Increased levels of wildtype alpha-synuclein in the cell leads to increased intracellular hydrogen peroxide levels and causes death of dopaminergic neurons in rat primary culture. Subsequently, oxidative stress has been directly linked with alpha-synuclein aggregation in vitro. This raises the question whether increased alpha-synuclein expression might be linked to higher susceptibility to PD and whether alpha-synuclein promoter polymorphisms are associated with PD. Here, two polymorphisms (-116C>G and -668T>C) of the alpha-synuclein promoter defining four haplotypes have been characterized in 315 German PD patients. The influence of the four haplotypes on gene expression was studied by CAT reporter gene assays in neuronal SK-N-AS cells. The -668C/-116G haplotype revealed significant higher CAT expression than the -668T/-116G or the -668T/-116C haplotype, respectively. Although the -668C/-116G haplotype was more common in PD patients, this difference was not significant.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Clinical & Experimental Neuroscience (Krüger Group)
Disciplines :
Genetics & genetic processes
Author, co-author :
Holzmann, C.
Krüger, Rejko ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Saecker, A. M. M. Vieira
Schmitt, I.
Schols, L.
Berger, K.
Riess, O.
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Title :
Polymorphisms of the alpha-synuclein promoter: expression analyses and association studies in Parkinson's disease.
Publication date :
Journal title :
Journal of Neural Transmission
Volume :
Issue :
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Peer reviewed :
Peer reviewed
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