Title : Building a kinetic model of trehalose biosynthesis in Saccharomyces cerevisiae. Language : English Author, co-author : Smallbone, Kieran [> >] Malys, Naglis [> >] Messiha, Hanan L. [> >] Wishart, Jill A. [> >] Simeonidis, Evangelos [University of Manchester > Manchester Centre for Integrative Systems Biology] Publication date : 2011 Journal title : Methods in Enzymology Volume : 500 Special issue title : Methods in Systems Biology Pages : 355-70 Peer reviewed : Yes (verified by ORBilu ) Audience : International ISSN : 0076-6879 Country : United States Keywords : [en] Algorithms ; Computer Simulation ; Enzyme Assays ; Gene Expression Regulation, Fungal ; Glucose-6-Phosphate Isomerase/chemistry/physiology ; Glucosyltransferases/chemistry/physiology ; Glycolysis ; Kinetics ; Metabolic Networks and Pathways ; Models, Biological ; Phosphoglucomutase/chemistry/physiology ; Saccharomyces cerevisiae/enzymology/metabolism/physiology ; Saccharomyces cerevisiae Proteins/chemistry/physiology ; Stress, Physiological ; Systems Biology ; Trehalose/biosynthesis ; UTP-Glucose-1-Phosphate Uridylyltransferase/chemistry/physiologyAbstract : [en] In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast. The biology of the trehalose cycle is briefly reviewed and discussed.Permalink : http://hdl.handle.net/10993/1663 DOI : 10.1016/B978-0-12-385118-5.00018-9 Other URL : http://www.sciencedirect.com/science/article/pii/B9780123851185000189 Commentary : Copyright (c) 2011 Elsevier Inc. All rights reserved. 
[University of Manchester > Manchester Centre for Integrative Systems Biology]
