Building a kinetic model of trehalose biosynthesis in Saccharomyces cerevisiae.

Algorithms; Computer Simulation; Enzyme Assays; Gene Expression Regulation, Fungal; Glucose-6-Phosphate Isomerase/chemistry/physiology; Glucosyltransferases/chemistry/physiology; Glycolysis; Kinetics; Metabolic Networks and Pathways; Models, Biological; Phosphoglucomutase/chemistry/physiology; Saccharomyces cerevisiae/enzymology/metabolism/physiology; Saccharomyces cerevisiae Proteins/chemistry/physiology; Stress, Physiological; Systems Biology; Trehalose/biosynthesis; UTP-Glucose-1-Phosphate Uridylyltransferase/chemistry/physiology

Abstract :

[en] In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast. The biology of the trehalose cycle is briefly reviewed and discussed.

Disciplines :

Life sciences: Multidisciplinary, general & others

Author, co-author :

Smallbone, Kieran

Malys, Naglis

Messiha, Hanan L.

Wishart, Jill A.

Simeonidis, Evangelos ; University of Manchester > Manchester Centre for Integrative Systems Biology

Language :

English

Title :

Building a kinetic model of trehalose biosynthesis in Saccharomyces cerevisiae.

Publication date :

2011

Journal title :

Methods in Enzymology

ISSN :

0076-6879

Publisher :

Elsevier, United States

Special issue title :

Methods in Systems Biology

Volume :

500

Pages :

355-70

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.sciencedirect.com/science/article/pii/B9780123851185000189

Commentary :

Available on ORBilu :

since 17 May 2013

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