Reference : Toward systems metabolic engineering in cyanobacteria: opportunities and bottlenecks.
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
Toward systems metabolic engineering in cyanobacteria: opportunities and bottlenecks.
Nogales, Juan [University of California at San Diego > Department of Bioengineering]
Gudmundsson, Steinn [University of Iceland > Center for Systems Biology]
Thiele, Ines mailto [University of Iceland > Center for Systems Biology]
United States
[en] COBRA methods ; Synechocystissp. PCC6803 ; biosustainability ; genome-scale modeling ; metabolic engineering ; photosynthetic robustness
[en] We recently assessed the metabolism of Synechocystis sp PCC6803 through a constraints-based reconstruction and analysis approach and identified its main metabolic properties. These include reduced metabolic robustness, in contrast to a high photosynthetic robustness driving the optimal autotrophic metabolism. Here, we address how these metabolic features affect biotechnological capabilities of this bacterium. The search for growth-coupled overproducer strains revealed that the carbon flux re-routing, but not the electron flux, is significantly more challenging under autotrophic conditions than under mixo- or heterotrophic conditions. We also found that the blocking of the light-driven metabolism was required for carbon flux re-routing under mixotrophic conditions. Overall, our analysis, which represents the first systematic evaluation of the biotechnological capabilities of a photosynthetic organism, paradoxically suggests that the light-driven metabolism itself and its unique metabolic features are the main bottlenecks in harnessing the biotechnological potential of Synechocystis.
The Spanish MEC through National Plan of I-D+i 2008–2011

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