No full text
Article (Scientific journals)
Characterization and developmental expression of Pax9, a paired-box-containing gene related to Pax1.
Neubuser, A.; Koseki, H.; Balling, Rudi
1995In Developmental Biology, 170 (2), p. 701-16
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Amino Acid Sequence; Animals; Cloning, Molecular; DNA/metabolism; DNA, Complementary/genetics; DNA-Binding Proteins/genetics/metabolism; Extremities/embryology; Female; Gene Expression Regulation, Developmental; In Situ Hybridization; Male; Mice; Mice, Inbred C57BL; Mice, Mutant Strains; Molecular Sequence Data; PAX9 Transcription Factor; Paired Box Transcription Factors; Protein Binding; Spine/embryology/metabolism; Thymus Gland/metabolism; Transcription Factors/genetics/metabolism
Abstract :
[en] Pax9, a recently identified mouse paired-box-containing gene, is highly homologous to Pax1 and belongs to the same subfamily as Pax1, Hup48, PAX9, and pox meso. Two overlapping cDNA clones spanning the entire coding region of Pax9 were isolated and sequenced. A comparison of the Pax1 and -9 protein sequences reveals a high degree of similarity even outside the paired box, while the carboxy-terminus of the two proteins diverges completely. We demonstrate that Pax9 can bind to the e5 sequence from the Drosophila even skipped promoter, which is also recognized by Pax1. We analyzed the expression of Pax9 during embryogenesis of wildtype, Undulated short-tail (Uns), and Danforth's short tail (Sd) mice. In wildtype embryos Pax9 is expressed in the pharyngeal pouches and their derivatives, the developing vertebral column, the tail, the head, and the limbs. Expression of Pax9 is unaffected in Uns embryos, in which the Pax1 gene is deleted, arguing that expression of Pax9 is not dependent on Pax1. The expression of Pax9 is lost in the caudal part of Sd homozygous embryos, suggesting that expression of Pax9 in the vertebral column is dependent on the notochord. These results indicate that both Pax9 and -1 may act in parallel during morphogenesis of the vertebral column.
Disciplines :
Genetics & genetic processes
Author, co-author :
Neubuser, A.
Koseki, H.
Balling, Rudi 
Language :
English
Title :
Characterization and developmental expression of Pax9, a paired-box-containing gene related to Pax1.
Publication date :
1995
Journal title :
Developmental Biology
ISSN :
1095-564X
Publisher :
Elsevier, Atlanta, United States - Florida
Volume :
170
Issue :
2
Pages :
701-16
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 10 May 2013

Statistics


Number of views
49 (0 by Unilu)
Number of downloads
0 (0 by Unilu)

Scopus citations®
 
248
Scopus citations®
without self-citations
227
OpenCitations
 
230
WoS citations
 
227

Bibliography


Similar publications



Contact ORBilu