Reference : Separate elements cause lineage restriction and specify boundaries of Hox-1.1 expression.
Scientific journals : Article
Life sciences : Genetics & genetic processes
Separate elements cause lineage restriction and specify boundaries of Hox-1.1 expression.
Puschel, A. W. [> >]
Balling, Rudi mailto [> >]
Gruss, P. [> >]
Company of Biologists
Yes (verified by ORBilu)
[en] Animals ; Gastrula/physiology/ultrastructure ; Gene Expression Regulation/genetics ; Genes, Homeobox/genetics ; Genetic Markers ; Lac Operon/physiology ; Mesoderm/physiology ; Mice ; Mice, Transgenic
[en] The Hox genes are a class of putative developmental control genes that are thought to be involved in the specification of positional identity along the anteroposterior axis of the vertebrate embryo. It is apparent from their expression pattern that their regulation is dependent upon positional information. In a previous analysis of the Hox-1.1 promoter in transgenic mice, we identified sequences that were sufficient to establish transgene expression in a specific region of the embryo. The construct used, however, did not contain enough regulatory sequences to reproduce all aspects of Hox-1.1 expression. In particular, neither a posterior boundary nor a restriction of expression to prevertebrae was achieved. Here we show correct regulation by Hox-1.1 sequences in transgenic mice and identify the elements responsible for different levels of control. Concomitant with the subdivision of mesodermal cells into different lineages during gastrulation and organogenesis, Hox-1.1 expression is restricted to successively smaller sets of cells. Distinct elements are required at different stages of development to execute this developmental programme. One position-responsive element (130 bp nontranslated leader) was shown to be crucial for the restriction of expression not only along the anteroposterior axis of the embryo, setting the posterior border, but also along the dorsoventral axis of the neural tube and to the lineage giving rise to the prevertebrae. Thus, Hox-1.1 expression is established in a specific region of the embryo and in a specific lineage of the mesoderm by restricting the activity of the promoter by the combined effect of several regulatory elements.

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