Reference : Tumorigenesis and eye abnormalities in transgenic mice expressing MSV-SV40 large T-an...
Scientific journals : Article
Life sciences : Genetics & genetic processes
Tumorigenesis and eye abnormalities in transgenic mice expressing MSV-SV40 large T-antigen.
Theuring, F. [> >]
Gotz, W. [> >]
Balling, Rudi mailto []
Korf, H. W. [> >]
Schulze, F. [> >]
Herken, R. [> >]
Gruss, P. [> >]
Yes (verified by ORBilu)
[en] Animals ; Antigens, Polyomavirus Transforming/analysis/genetics ; Brain Neoplasms/etiology ; Chromosome Deletion ; Enhancer Elements, Genetic ; Eye/pathology ; Eye Abnormalities/genetics ; Female ; Genotype ; Male ; Mice ; Mice, Transgenic ; Moloney murine sarcoma virus/genetics ; Neoplasms, Experimental/genetics ; Phenotype ; Pineal Gland/immunology/pathology ; Sarcoma Viruses, Murine/genetics ; Simian virus 40/genetics
[en] Transgenic mice which expressed SV40 large T-antigen under the control of the MSV enhancer and the SV40 promoter were generated. In animals containing an intact MSV enhancer, total lens cataracts and neuroectodermal brain tumors, originating in the pineal organ were observed. In contrast, 5' deletion of the MSV enhancer to a residual 53 bp resulted in a different spectrum of pathologies. Whilst lens cataracts still occurred, no brain tumors could be detected. Instead, fibrosarcomas and adenocarcinomas of the kidneys were induced. In addition, tumors of the endocrine pancreas were observed with both transgene constructs. We conclude that the MSV enhancer element is sufficient to direct the expression of the viral reporter gene to the lens and the pineal organ in transgenic mice. Deletion of the MSV enhancer correlates with the loss of DNA elements responsible for the pineal cell specific expression of SV40 large T-antigen.

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