Article (Scientific journals)
Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins.
Haan, Claude; Haan, Serge
2013In Methods in Molecular Biology, 967, p. 21-38
Peer reviewed
 

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Abstract :
[en] Jak binding to cytokine receptors has been shown to be a complex and tight interaction. When studying loss-of-function or gain-of-function mutants of the Jaks or cytokine receptors it is often necessary to know if a certain mutant still associates correctly in the context of the signaling complex. The standard technique to show interaction of Jaks with cytokine receptors or other signalling molecules is Co-immunoprecipitation. Here we describe our protocol and discuss different pitfalls that can be encountered during the procedure.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Haan, Claude ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Haan, Serge ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
External co-authors :
no
Language :
English
Title :
Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins.
Publication date :
2013
Journal title :
Methods in Molecular Biology
ISSN :
1064-3745
Volume :
967
Pages :
21-38
Peer reviewed :
Peer reviewed
Available on ORBilu :
since 13 November 2013

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