Article (Scientific journals)
Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents.
Haan, Serge; Haan, Claude
2013In Methods in Molecular Biology, 967, p. 147-59
Peer reviewed
 

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Abstract :
[en] Here we describe the preparation of nuclear extracts and the electrophoretic mobility shift assay (EMSA) for the detection of STAT species. We use the method for the investigation of STAT1 and STAT3 homo- and heterodimers and show how the preparation of the extracts can influence the distribution of the STAT species observed in the EMSA. We show that detergents can massively influence the STAT dimer distribution. Although it is unclear whether they primarily interfere with STAT DNA binding and/or whether they break up or further oligomerize STATs, the observation may also have an impact on the results of other techniques performed with detergent-containing cell lysates (e.g., coimmunoprecipitations of STATs with other proteins).
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Haan, Serge ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Haan, Claude ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
External co-authors :
no
Language :
English
Title :
Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents.
Publication date :
2013
Journal title :
Methods in Molecular Biology
ISSN :
1064-3745
Volume :
967
Pages :
147-59
Peer reviewed :
Peer reviewed
Available on ORBilu :
since 13 November 2013

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