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Correlation between growth rates, EIIACrr phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12.
Bettenbrock, Katja; SAUTER, Thomas; Jahreis, Knut et al.
2007In Journal of Bacteriology, 189 (19), p. 6891-900
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Keywords :
Bioreactors/microbiology; Cyclic AMP/metabolism; Cyclic AMP Receptor Protein/genetics/metabolism; Escherichia coli/genetics/growth & development/metabolism; Escherichia coli Proteins/genetics/metabolism; Gene Expression Regulation, Bacterial; Glucose/metabolism; Mannitol/metabolism; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics/metabolism; Phosphorylation; Promoter Regions, Genetic/genetics; Sucrose/metabolism
Abstract :
[en] In Escherichia coli K-12, components of the phosphoenolpyruvate-dependent phosphotransferase systems (PTSs) represent a signal transduction system involved in the global control of carbon catabolism through inducer exclusion mediated by phosphoenolpyruvate-dependent protein kinase enzyme IIA(Crr) (EIIA(Crr)) (= EIIA(Glc)) and catabolite repression mediated by the global regulator cyclic AMP (cAMP)-cAMP receptor protein (CRP). We measured in a systematic way the relation between cellular growth rates and the key parameters of catabolite repression, i.e., the phosphorylated EIIA(Crr) (EIIA(Crr) approximately P) level and the cAMP level, using in vitro and in vivo assays. Different growth rates were obtained by using either various carbon sources or by growing the cells with limited concentrations of glucose, sucrose, and mannitol in continuous bioreactor experiments. The ratio of EIIA(Crr) to EIIA(Crr) approximately P and the intracellular cAMP concentrations, deduced from the activity of a cAMP-CRP-dependent promoter, correlated well with specific growth rates between 0.3 h(-1) and 0.7 h(-1), corresponding to generation times of about 138 and 60 min, respectively. Below and above this range, these parameters were increasingly uncoupled from the growth rate, which perhaps indicates an increasing role executed by other global control systems, in particular the stringent-relaxed response system.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Bettenbrock, Katja
SAUTER, Thomas ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Jahreis, Knut
Kremling, Andreas
Lengeler, Joseph W.
Gilles, Ernst-Dieter
Language :
English
Title :
Correlation between growth rates, EIIACrr phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12.
Publication date :
2007
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
189
Issue :
19
Pages :
6891-900
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 26 April 2013

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