[en] Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems.
Disciplines :
Sciences de l’environnement & écologie
Identifiants :
UNILU:UL-ARTICLE-2011-684
Auteur, co-auteur :
Knierim, B.
Luef, B.
WILMES, Paul ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Webb, R. I.
Auer, Manfred
Comolli, L. R.
Banfield, J. F.
Co-auteurs externes :
yes
Langue du document :
Anglais
Titre :
Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities
