Article (Scientific journals)
Metaproteomics Provides Functional Insight into Activated Sludge Wastewater Treatment
Wilmes, Paul; Wexler, Margaret; Bond, Philip L.
2008In PLoS ONE, 3 (3), p. 1-2
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Abstract :
[en] Background: Through identification of highly expressed proteins from a mixed culture activated sludge system this study provides functional evidence of microbial transformations important for enhanced biological phosphorus removal (EBPR). Methodology/Principal Findings: A laboratory-scale sequencing batch reactor was successfully operated for different levels of EBPR, removing around 25, 40 and 55 mg/l P. The microbial communities were dominated by the uncultured polyphosphate-accumulating organism "Candidatus Accumulibacter phosphatis''. When EBPR failed, the sludge was dominated by tetrad-forming alpha-Proteobacteria. Representative and reproducible 2D gel protein separations were obtained for all sludge samples. 638 protein spots were matched across gels generated from the phosphate removing sludges. 111 of these were excised and 46 proteins were identified using recently available sludge metagenomic sequences. Many of these closely match proteins from "Candidatus Accumulibacter phosphatis'' and could be directly linked to the EBPR process. They included enzymes involved in energy generation, polyhydroxyalkanoate synthesis, glycolysis, gluconeogenesis, glycogen synthesis, glyoxylate/TCA cycle, fatty acid beta oxidation, fatty acid synthesis and phosphate transport. Several proteins involved in cellular stress response were detected. Conclusions/Significance: Importantly, this study provides direct evidence linking the metabolic activities of "Accumulibacter'' to the chemical transformations observed in EBPR. Finally, the results are discussed in relation to current EBPR metabolic models.
Disciplines :
Environmental sciences & ecology
Identifiers :
UNILU:UL-ARTICLE-2011-693
Author, co-author :
Wilmes, Paul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Wexler, Margaret
Bond, Philip L.
External co-authors :
yes
Language :
English
Title :
Metaproteomics Provides Functional Insight into Activated Sludge Wastewater Treatment
Publication date :
2008
Journal title :
PLoS ONE
ISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
3
Issue :
3
Pages :
1-2
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 07 October 2013

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