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Characterization and binding specificity of the monomeric STAT3-SH2 domain.
Haan, Serge; Hemmann, U.; Hassiepen, U. et al.
1999In Journal of Biological Chemistry, 274 (3), p. 1342-8
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Keywords :
Binding Sites; Circular Dichroism; DNA-Binding Proteins/metabolism; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Phosphotyrosine/metabolism; Protein Conformation; Protein Folding; STAT3 Transcription Factor; Spectrometry, Fluorescence; Trans-Activators/metabolism; src Homology Domains
Abstract :
[en] Signal transducers and activators of transcription (STATs) are important mediators of cytokine signal transduction. STAT factors are recruited to phosphotyrosine-containing motifs of activated receptor chains via their SH2 domains. The subsequent tyrosine phosphorylation of the STATs leads to their dissociation from the receptor, dimerization, and translocation to the nucleus. Here we describe the expression, purification, and refolding of the STAT3-SH2 domain. Proper folding of the isolated protein was proven by circular dichroism and fluorescence spectroscopy. The STAT3-SH2 domain undergoes a conformational change upon dimerization. Using an enzyme-linked immunosorbent assay we demonstrate that the monomeric domain binds to specific phosphotyrosine peptides. The specificity of binding to phosphotyrosine peptides was assayed with the tyrosine motif encompassing Tyr705 of STAT3 and with all tyrosine motifs present in the cytoplasmic tail of the signal transducer gp130.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Haan, Serge ;  Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > Institute for Biochemistry
Hemmann, U.
Hassiepen, U.
Schaper, F.
Schneider-Mergener, J.
Wollmer, A.
Heinrich, P. C.
Grotzinger, J.
External co-authors :
yes
Language :
English
Title :
Characterization and binding specificity of the monomeric STAT3-SH2 domain.
Publication date :
1999
Journal title :
Journal of Biological Chemistry
ISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology, United States - Maryland
Volume :
274
Issue :
3
Pages :
1342-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 25 April 2013

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