Actins/metabolism; Animals; Antibodies/chemistry/genetics/metabolism; Antibody Affinity; Camelids, New World/genetics/immunology; Cell Line, Tumor; Cell Movement/immunology/physiology; Humans; Immunoglobulin Heavy Chains/chemistry/genetics/metabolism; Immunoglobulin Variable Region/chemistry/genetics/metabolism; Male; Microfilament Proteins/antagonists & inhibitors/genetics/immunology/metabolism; Neoplasm Invasiveness/immunology/physiopathology; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Pseudopodia/immunology/metabolism; RNA Interference; Recombinant Proteins/chemistry/genetics/metabolism; Thermodynamics
Abstract :
[en] L-plastin, a conserved modular F-actin bundling protein, is ectopically expressed in tumor cells and contributes to cell malignancy and invasion. The underlying molecular mechanisms involved remain unclear, in part, because specific inhibitors of L-plastin are lacking. We used recombinant alpaca-derived L-plastin single-domain antibodies (nanobodies) as effector of L-plastin function in cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Delanote, Veerle
Vanloo, Berlinda
Catillon, Marie ; University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Friederich, Evelyne ; University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Vandekerckhove, Joel
Gettemans, Jan
Language :
English
Title :
An alpaca single-domain antibody blocks filopodia formation by obstructing L-plastin-mediated F-actin bundling.
Publication date :
2010
Journal title :
FASEB Journal
ISSN :
1530-6860
Publisher :
Federation of American Societies for Experimental Biology, United States - Maryland