[en] Actins/metabolism ; Animals ; Antibodies/chemistry/genetics/metabolism ; Antibody Affinity ; Camelids, New World/genetics/immunology ; Cell Line, Tumor ; Cell Movement/immunology/physiology ; Humans ; Immunoglobulin Heavy Chains/chemistry/genetics/metabolism ; Immunoglobulin Variable Region/chemistry/genetics/metabolism ; Male ; Microfilament Proteins/antagonists & inhibitors/genetics/immunology/metabolism ; Neoplasm Invasiveness/immunology/physiopathology ; Protein Binding ; Protein Conformation ; Protein Structure, Tertiary ; Pseudopodia/immunology/metabolism ; RNA Interference ; Recombinant Proteins/chemistry/genetics/metabolism ; Thermodynamics
[en] L-plastin, a conserved modular F-actin bundling protein, is ectopically expressed in tumor cells and contributes to cell malignancy and invasion. The underlying molecular mechanisms involved remain unclear, in part, because specific inhibitors of L-plastin are lacking. We used recombinant alpaca-derived L-plastin single-domain antibodies (nanobodies) as effector of L-plastin function in cells.
[University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
