Keywords :
Actins/metabolism; Animals; Antibodies/chemistry/genetics/metabolism; Antibody Affinity; Camelids, New World/genetics/immunology; Cell Line, Tumor; Cell Movement/immunology/physiology; Humans; Immunoglobulin Heavy Chains/chemistry/genetics/metabolism; Immunoglobulin Variable Region/chemistry/genetics/metabolism; Male; Microfilament Proteins/antagonists & inhibitors/genetics/immunology/metabolism; Neoplasm Invasiveness/immunology/physiopathology; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Pseudopodia/immunology/metabolism; RNA Interference; Recombinant Proteins/chemistry/genetics/metabolism; Thermodynamics
Abstract :
[en] L-plastin, a conserved modular F-actin bundling protein, is ectopically expressed in tumor cells and contributes to cell malignancy and invasion. The underlying molecular mechanisms involved remain unclear, in part, because specific inhibitors of L-plastin are lacking. We used recombinant alpaca-derived L-plastin single-domain antibodies (nanobodies) as effector of L-plastin function in cells.
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without self-citations
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