[en] The gut microbiome comprises trillions of microorganisms and profoundly influences human health by modulating metabolism, immune responses and neuronal functions. Disruption in gut microbiome composition is implicated in various inflammatory conditions, metabolic disorders and neurodegenerative diseases. However, determining the underlying mechanisms and establishing cause and effect is extremely difficult. Preclinical models offer crucial insights into the role of the gut microbiome in diseases and help identify potential therapeutic interventions. The Human Microbiome Action Consortium initiated a Delphi survey to assess the utility of preclinical models, including animal and cell-based models, in elucidating the causal role of the gut microbiome in these diseases. The Delphi survey aimed to address the complexity of selecting appropriate preclinical models to investigate disease causality and to study host-microbiome interactions effectively. We adopted a structured approach encompassing a literature review, expert workshops and the Delphi questionnaire to gather insights from a diverse range of stakeholders. Experts were requested to evaluate the strengths, limitations, and suitability of these models in addressing the causal relationship between the gut microbiome and disease pathogenesis. The resulting consensus statements and recommendations provide valuable insights for selecting preclinical models in future studies of gut microbiome-related diseases.
Disciplines :
Microbiology
Author, co-author :
Metwaly, Amira ; Chair of Nutrition and Immunology, TUM School of Life Sciences, Technical University Munich, Freising, Germany ; ZIEL Institute for Food & Health, Technical University Munich, Freising, Germany
Kriaa, Aicha; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France
Hassani, Zahra; KPL-Paris, Paris, France
Carraturo, Federica; European Biomedical Research Institute of Salerno (EBRIS), Salerno, Italy
Druart, Celine ; Pharmabiotic Research Institute, Narbonne, France
IHMCSA Consortium
Arnauts, Kaline; Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, Leuven, Belgium
WILMES, Paul ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology
Walter, Jens ; APC Microbiome Ireland, School of Microbiology, and Department of Medicine, University College Cork, Cork, Ireland
Rosshart, Stephan ; Department of Microbiome Research, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany ; Department of Medicine II, Medical Center - University of Freiburg, Faculty of Medicine, Freiburg, Germany
DESAI, Mahesh ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine > Research LCSB ; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg
Dore, Joel ; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France ; Université Paris-Saclay, INRAE, MetaGenoPolis, Jouy-en-Josas, France
Fasano, Alessio; European Biomedical Research Institute of Salerno (EBRIS), Salerno, Italy ; Department of Paediatric Gastroenterology and Nutrition, Mucosal Immunology and Biology Research Center,Massachusetts General Hospital Brigham, Harvard Medical School, Boston, MA, USA
Blottiere, Hervé M ; Université Paris-Saclay, INRAE, MetaGenoPolis, Jouy-en-Josas, France ; Nantes Université, INRAE, UMR1280, PhAN, Nantes, France
Maguin, Emmanuelle ; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France. emmanuelle.maguin@inrae.fr
Haller, Dirk ; Chair of Nutrition and Immunology, TUM School of Life Sciences, Technical University Munich, Freising, Germany. dirk.haller@tum.de ; ZIEL Institute for Food & Health, Technical University Munich, Freising, Germany. dirk.haller@tum.de
This Delphi survey was conducted within the framework of the International Human Microbiome Coordination and Support Action (IHMCSA) project supported by funding from the European Union\u2019s Horizon 2020 research and innovation program under grant agreement number 964590. The authors received funding from Deutsche Forschungsgemeinschaft (DFG, German Research Foundation), project number 395357507 (SFB 1371, Microbiome Signatures).
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