No full text
Article (Scientific journals)
Growing an actin gel on spherical surfaces.
Noireaux, V.; Golsteyn, R. M.; Friederich, Evelyne et al.
2000In Biophysical journal, 78 (3), p. 1643-54
Peer reviewed


Full Text
No document available.

Send to


Keywords :
Actins/chemistry/physiology/ultrastructure; Animals; Bacterial Proteins/chemistry/physiology/ultrastructure; Cell-Free System; Cytosol/physiology; Gels; Glutathione Transferase/chemistry; HeLa Cells; Humans; Listeria monocytogenes/physiology/ultrastructure; Membrane Proteins/chemistry/physiology/ultrastructure; Microscopy, Electron; Microscopy, Immunoelectron; Models, Biological; Movement; Muscle, Skeletal; Rabbits; Recombinant Fusion Proteins/chemistry/ultrastructure; Surface Properties
Abstract :
[en] Inspired by the motility of the bacteria Listeria monocytogenes, we have experimentally studied the growth of an actin gel around spherical beads grafted with ActA, a protein known to be the promoter of bacteria movement. On ActA-grafted beads F-actin is formed in a spherical manner, whereas on the bacteria a "comet-like" tail of F-actin is produced. We show experimentally that the stationary thickness of the gel depends on the radius of the beads. Moreover, the actin gel is not formed if the ActA surface density is too low. To interpret our results, we propose a theoretical model to explain how the mechanical stress (due to spherical geometry) limits the growth of the actin gel. Our model also takes into account treadmilling of actin. We deduce from our work that the force exerted by the actin gel on the bacteria is of the order of 10 pN. Finally, we estimate from our theoretical model possible conditions for developing actin comet tails.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Noireaux, V.
Golsteyn, R. M.
Friederich, Evelyne ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Prost, J.
Antony, C.
Louvard, D.
Sykes, C.
Language :
Title :
Growing an actin gel on spherical surfaces.
Publication date :
Journal title :
Biophysical journal
Volume :
Issue :
Pages :
Peer reviewed :
Peer reviewed
Available on ORBilu :
since 18 September 2013


Number of views
59 (0 by Unilu)
Number of downloads
0 (0 by Unilu)

Scopus citations®
Scopus citations®
without self-citations
WoS citations


Similar publications

Contact ORBilu