Reference : ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity. |
Scientific journals : Article | |||
Life sciences : Biochemistry, biophysics & molecular biology | |||
http://hdl.handle.net/10993/6277 | |||
ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity. | |
English | |
Fradelizi, J. [> >] | |
Noireaux, V. [> >] | |
Plastino, J. [> >] | |
Menichi, B. [> >] | |
Louvard, D. [> >] | |
Sykes, C. [> >] | |
Golsteyn, R. M. [> >] | |
Friederich, Evelyne ![]() | |
2001 | |
Nature Cell Biology | |
3 | |
8 | |
699-707 | |
Yes (verified by ORBilu) | |
International | |
1465-7392 | |
England | |
[en] Actin Cytoskeleton/drug effects/metabolism/ultrastructure ; Actin-Related Protein 2 ; Actin-Related Protein 3 ; Actins/metabolism ; Animals ; Bacterial Proteins/metabolism ; Biological Assay ; Cell Adhesion Molecules/metabolism ; Cell-Free System ; Cercopithecus aethiops ; Cytoskeletal Proteins ; Fluorescent Antibody Technique ; Glycoproteins ; HeLa Cells/cytology/drug effects/metabolism ; Humans ; Membrane Proteins/metabolism ; Metalloproteins/genetics/metabolism ; Microfilament Proteins ; Microspheres ; Mitochondria/metabolism/ultrastructure ; Phosphoproteins/metabolism ; Polymers/metabolism ; Proteins/metabolism ; Recombinant Proteins/metabolism ; Transfection ; Vero Cells/cytology/drug effects/metabolism ; Wiskott-Aldrich Syndrome Protein ; Zyxin | |
[en] The actin cytoskeleton is a dynamic network that is composed of a variety of F-actin structures. To understand how these structures are produced, we tested the capacity of proteins to direct actin polymerization in a bead assay in vitro and in a mitochondrial-targeting assay in cells. We found that human zyxin and the related protein ActA of Listeria monocytogenes can generate new actin structures in a vasodilator-stimulated phosphoprotein-dependent (VASP) manner, but independently of the Arp2/3 complex. These results are consistent with the concept that there are multiple actin-polymerization machines in cells. With these simple tests it is possible to probe the specific function of proteins or identify novel molecules that act upon cellular actin polymerization. | |
http://hdl.handle.net/10993/6277 | |
10.1038/35087009 |
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