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Article (Scientific journals)
ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity.
Fradelizi, J.; Noireaux, V.; Plastino, J. et al.
2001In Nature Cell Biology, 3 (8), p. 699-707
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Keywords :
Actin Cytoskeleton/drug effects/metabolism/ultrastructure; Actin-Related Protein 2; Actin-Related Protein 3; Actins/metabolism; Animals; Bacterial Proteins/metabolism; Biological Assay; Cell Adhesion Molecules/metabolism; Cell-Free System; Cercopithecus aethiops; Cytoskeletal Proteins; Fluorescent Antibody Technique; Glycoproteins; HeLa Cells/cytology/drug effects/metabolism; Humans; Membrane Proteins/metabolism; Metalloproteins/genetics/metabolism; Microfilament Proteins; Microspheres; Mitochondria/metabolism/ultrastructure; Phosphoproteins/metabolism; Polymers/metabolism; Proteins/metabolism; Recombinant Proteins/metabolism; Transfection; Vero Cells/cytology/drug effects/metabolism; Wiskott-Aldrich Syndrome Protein; Zyxin
Abstract :
[en] The actin cytoskeleton is a dynamic network that is composed of a variety of F-actin structures. To understand how these structures are produced, we tested the capacity of proteins to direct actin polymerization in a bead assay in vitro and in a mitochondrial-targeting assay in cells. We found that human zyxin and the related protein ActA of Listeria monocytogenes can generate new actin structures in a vasodilator-stimulated phosphoprotein-dependent (VASP) manner, but independently of the Arp2/3 complex. These results are consistent with the concept that there are multiple actin-polymerization machines in cells. With these simple tests it is possible to probe the specific function of proteins or identify novel molecules that act upon cellular actin polymerization.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Fradelizi, J.
Noireaux, V.
Plastino, J.
Menichi, B.
Louvard, D.
Sykes, C.
Golsteyn, R. M.
FRIEDERICH, Evelyne ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Language :
English
Title :
ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity.
Publication date :
2001
Journal title :
Nature Cell Biology
ISSN :
1465-7392
eISSN :
1476-4679
Publisher :
Nature Publishing Group, United Kingdom
Volume :
3
Issue :
8
Pages :
699-707
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 18 September 2013

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