Reference : A single amino acid substitution (Trp(666)-->Ala) in the interbox1/2 region of the in... |
Scientific journals : Article | |||
Life sciences : Biochemistry, biophysics & molecular biology | |||
http://hdl.handle.net/10993/6243 | |||
A single amino acid substitution (Trp(666)-->Ala) in the interbox1/2 region of the interleukin-6 signal transducer gp130 abrogates binding of JAK1, and dominantly impairs signal transduction | |
English | |
Haan, Claude ![]() | |
Hermanns, H. M. [> >] | |
Heinrich, P. C. [> >] | |
Behrmann, Iris ![]() | |
2001 | |
Biochemical Journal | |
Portland Press | |
349 | |
Pt 1 | |
261-6 | |
Yes (verified by ORBilu) | |
International | |
0264-6021 | |
1470-8728 | |
London | |
United Kingdom | |
[en] Alanine ; Mutagenesis, Site-Directed ; Point Mutation ; Protein Binding ; Protein Structure, Tertiary ; Protein-Tyrosine Kinases ; Receptors, Interleukin ; Receptors, Interleukin-5 ; Signal Transduction ; Trans-Activation (Genetics) ; Transfection ; Mice ; Membrane Glycoproteins ; Animals ; Antigens, CD ; B-Lymphocytes ; COS Cells ; Cell Division ; Cytokine Receptor gp130 ; Cytoplasm ; Dose-Response Relationship, Drug ; Interleukin-6 ; Janus Kinase 1 ; Tryptophan | |
[en] gp130 is the common signal-transducing receptor chain of interleukin (IL)-6-type cytokines. Here we describe, for the first time, a single amino acid substitution (Trp(666)-->Ala) in the membrane-proximal interbox1/2 region that abrogates activation of STAT (signal transducer and activator of transcription) transcription factors and the proliferative response of pro-B-cell transfectants. Moreover, association of the Janus kinase JAK1 is prevented. No signalling of heterodimeric IL-5 receptor (IL-5R)/gp130 chimaeras occurs in COS-7 cells, even when only a single cytoplasmic chain of a gp130 dimer contains the Trp(666)Ala mutation, indicating that it acts dominantly. | |
http://hdl.handle.net/10993/6243 |
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