Reference : A single amino acid substitution (Trp(666)-->Ala) in the interbox1/2 region of the in...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/6243
A single amino acid substitution (Trp(666)-->Ala) in the interbox1/2 region of the interleukin-6 signal transducer gp130 abrogates binding of JAK1, and dominantly impairs signal transduction
English
Haan, Claude mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Hermanns, H. M. [> >]
Heinrich, P. C. [> >]
Behrmann, Iris mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
2001
Biochemical Journal
Portland Press
349
Pt 1
261-6
Yes (verified by ORBilu)
International
0264-6021
1470-8728
London
United Kingdom
[en] Alanine ; Mutagenesis, Site-Directed ; Point Mutation ; Protein Binding ; Protein Structure, Tertiary ; Protein-Tyrosine Kinases ; Receptors, Interleukin ; Receptors, Interleukin-5 ; Signal Transduction ; Trans-Activation (Genetics) ; Transfection ; Mice ; Membrane Glycoproteins ; Animals ; Antigens, CD ; B-Lymphocytes ; COS Cells ; Cell Division ; Cytokine Receptor gp130 ; Cytoplasm ; Dose-Response Relationship, Drug ; Interleukin-6 ; Janus Kinase 1 ; Tryptophan
[en] gp130 is the common signal-transducing receptor chain of interleukin (IL)-6-type cytokines. Here we describe, for the first time, a single amino acid substitution (Trp(666)-->Ala) in the membrane-proximal interbox1/2 region that abrogates activation of STAT (signal transducer and activator of transcription) transcription factors and the proliferative response of pro-B-cell transfectants. Moreover, association of the Janus kinase JAK1 is prevented. No signalling of heterodimeric IL-5 receptor (IL-5R)/gp130 chimaeras occurs in COS-7 cells, even when only a single cytoplasmic chain of a gp130 dimer contains the Trp(666)Ala mutation, indicating that it acts dominantly.
http://hdl.handle.net/10993/6243

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