Reference : A region encompassing the FERM domain of Jak1 is necessary for binding to the cytokin...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
A region encompassing the FERM domain of Jak1 is necessary for binding to the cytokine receptor gp130
Hilkens, C. M. [> >]
Isharc, H. [> >]
Lillemeier, B. F. [> >]
Strobl, B. [> >]
Bates, P. A. [> >]
Behrmann, Iris mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Kerr, I. M. [> >]
FEBS Letters
Elsevier Science
Yes (verified by ORBilu)
The Netherlands
[en] Tumor Cells, Cultured ; Antigens, CD ; Binding Sites ; Cytokine Receptor gp130 ; Cytoplasm ; DNA-Binding Proteins ; Fibrosarcoma ; Humans ; Janus Kinase 1 ; Janus Kinase 3 ; Membrane Glycoproteins ; Molecular Sequence Data ; Protein Structure, Tertiary ; Protein-Tyrosine Kinases ; Recombinant Proteins ; STAT1 Transcription Factor ; Signal Transduction ; Trans-Activators ; Amino Acid Sequence
[en] The terminal portion of the Janus kinases (Jaks) contains a divergent FERM (Four-point-one, Ezrin, Radixin, Moesin) homology domain comprising 19 conserved hydrophobic regions. To determine the role of this domain in governing recruitment of Jak1, but not Jak3, to the gp130 subunit of the interleukin-6 family of cytokine receptors, the interaction of three Jak1/Jak3 chimeras with gp130 was investigated. Chimeras 1, 2 and 3 (Jak1 FERM regions 1-19, 1-18 and 1-8/Jak3, respectively) were all enzymically active. Chimeras 1 and 2 interacted with the cytoplasmic domain of gp130, although less efficiently than Jak1. Only chimera 2, however, restored gp130 signalling in Jak1-negative cells. The data are consistent with recruitment of Jak1 to gp130 through the Jak1 FERM domain, but also emphasise the likely requirement for precise Jak/receptor orientation to sustain function.

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