| Reference : A region encompassing the FERM domain of Jak1 is necessary for binding to the cytokin... |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/10993/6242 | |||
| A region encompassing the FERM domain of Jak1 is necessary for binding to the cytokine receptor gp130 | |
| English | |
| Hilkens, C. M. [> >] | |
| Isharc, H. [> >] | |
| Lillemeier, B. F. [> >] | |
| Strobl, B. [> >] | |
| Bates, P. A. [> >] | |
Behrmann, Iris  [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| Kerr, I. M. [> >] | |
| 2001 | |
| FEBS Letters | |
| Elsevier Science | |
| 505 | |
| 1 | |
| 87-91 | |
| Yes (verified by ORBilu) | |
| 0014-5793 | |
| Amsterdam | |
| The Netherlands | |
| [en] Tumor Cells, Cultured ; Antigens, CD ; Binding Sites ; Cytokine Receptor gp130 ; Cytoplasm ; DNA-Binding Proteins ; Fibrosarcoma ; Humans ; Janus Kinase 1 ; Janus Kinase 3 ; Membrane Glycoproteins ; Molecular Sequence Data ; Protein Structure, Tertiary ; Protein-Tyrosine Kinases ; Recombinant Proteins ; STAT1 Transcription Factor ; Signal Transduction ; Trans-Activators ; Amino Acid Sequence | |
| [en] The terminal portion of the Janus kinases (Jaks) contains a divergent FERM (Four-point-one, Ezrin, Radixin, Moesin) homology domain comprising 19 conserved hydrophobic regions. To determine the role of this domain in governing recruitment of Jak1, but not Jak3, to the gp130 subunit of the interleukin-6 family of cytokine receptors, the interaction of three Jak1/Jak3 chimeras with gp130 was investigated. Chimeras 1, 2 and 3 (Jak1 FERM regions 1-19, 1-18 and 1-8/Jak3, respectively) were all enzymically active. Chimeras 1 and 2 interacted with the cytoplasmic domain of gp130, although less efficiently than Jak1. Only chimera 2, however, restored gp130 signalling in Jak1-negative cells. The data are consistent with recruitment of Jak1 to gp130 through the Jak1 FERM domain, but also emphasise the likely requirement for precise Jak/receptor orientation to sustain function. | |
| http://hdl.handle.net/10993/6242 |
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