[en] It is crucial to understand the glucose control within our bodies. Bariatric/metabolic surgeries, including laparoscopic sleeve gastrectomy (LSG) and Roux-en-Y gastric bypass (RYGB), provide an avenue for exploring the potential key factors involved in maintaining glucose homeostasis since these surgeries have shown promising results in improving glycemic control among patients with severe type 2 diabetes (T2D). For the first time, a markedly altered population of serum proteins in patients after LSG was discovered and analyzed through proteomics. Apolipoprotein A-IV (apoA-IV) was revealed to be increased dramatically in diabetic obese patients following LSG, and a similar effect was observed in patients after RYGB surgery. Moreover, recombinant apoA-IV protein treatment was proven to enhance insulin secretion in isolated human islets. These results showed that apoA-IV may play a crucial role in glycemic control in humans, potentially through enhancing insulin secretion in human islets. ApoA-IV was further shown to enhance energy expenditure and improve glucose tolerance in diabetic rodents, through stimulating glucose-dependent insulin secretion in pancreatic β cells, partially via Gαs-coupled GPCR/cAMP (G protein-coupled receptor/cyclic adenosine monophosphate) signaling. Furthermore, T55−121, truncated peptide 55−121 of apoA-IV, was discovered to mediate the function of apoA-IV. These collective findings contribute to our understanding of the relationship between apoA-IV and glycemic control, highlighting its potential as a biomarker or therapeutic target in managing and improving glucose regulation.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Cao, Zhen ✱; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics , Chinese Academy of Sciences, Beijing 100101, China ; University of Chinese Academy of Sciences , Beijing 100049, China
Lei, Lei ✱; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics , Chinese Academy of Sciences, Beijing 100101, China ; The State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050, China
ZHOU, Ziyun ✱; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Gene Expression and Metabolism
Xu, Shimeng; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics , Chinese Academy of Sciences, Beijing 100101, China
Wang, Linlin; Guangzhou Regenerative Medicine and Health Guangdong Laboratory (Bioland Laboratory), Guangzhou , Guangdong 510005, China
Gong, Weikang; Department of Computer Science, School of Computing, National University of Singapore , Singapore 117417, Singapore
Zhang, Qi; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics , Chinese Academy of Sciences, Beijing 100101, China ; University of Chinese Academy of Sciences , Beijing 100049, China
Pan, Bin; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics , Chinese Academy of Sciences, Beijing 100101, China ; University of Chinese Academy of Sciences , Beijing 100049, China
Zhang, Gaoxin; School of Basic Medical Sciences, Southwest Medical University, Luzhou , Sichuan 646000, China
Yuan, Quan; School of Basic Medical Sciences, Southwest Medical University, Luzhou , Sichuan 646000, China
Cui, Liujuan; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics , Chinese Academy of Sciences, Beijing 100101, China
Zheng, Min ; The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China
Xu, Tao; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics , Chinese Academy of Sciences, Beijing 100101, China ; University of Chinese Academy of Sciences , Beijing 100049, China ; Guangzhou Regenerative Medicine and Health Guangdong Laboratory (Bioland Laboratory), Guangzhou , Guangdong 510005, China
Wang, You; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics , Chinese Academy of Sciences, Beijing 100101, China
Zhang, Shuyan; Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital , Capital Medical University, Beijing 100015, China ; Beijing Institute of Infectious Diseases , Beijing 100015, China ; National Center for Infectious Diseases, Beijing Ditan Hospital, Capital Medical University , Beijing 100015, China ; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases , Beijing 100015, China
Liu, Pingsheng; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics , Chinese Academy of Sciences, Beijing 100101, China ; University of Chinese Academy of Sciences , Beijing 100049, China
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