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Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen
Oehm, A.; Behrmann, Iris; Falk, W. et al.
1992In Journal of Biological Chemistry, 267 (15), p. 10709-15
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Keywords :
Amino Acid Sequence; Humans; Membrane Proteins; Molecular Sequence Data; Multigene Family; Nerve Growth Factors; Receptors, Cell Surface; Receptors, Nerve Growth Factor; Receptors, Tumor Necrosis Factor; Sequence Homology, Nucleic Acid; Enzyme-Linked Immunosorbent Assay; Electrophoresis, Polyacrylamide Gel; Antigens, CD95; Antigens, Neoplasm; Antigens, Surface; Base Sequence; Blotting, Western; Cell Death; Cloning, Molecular; Cross-Linking Reagents; DNA; Tumor Necrosis Factor-alpha
Abstract :
[en] The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 was previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. Cross-linking of the APO-1 antigen by anti-APO-1 induced programmed cell death, apoptosis, of APO-1 positive cells. To characterize the APO-1 cell surface molecule and to better understand its role in induction of apoptosis, the APO-1 protein was purified to homogeneity from membranes of SKW6.4 B lymphoblastoid cells by solubilization with sodium deoxycholate, affinity chromatography with anti-APO-1 antibody, and reversed phase high performance liquid chromatography. Each purification step was followed by an APO-1-specific solid phase enzyme-linked immunosorbent assay using the monoclonal antibody anti-APO-1. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the APO-1 antigen was found to be a membrane glycoprotein of 48-kDa. Endoproteinase-cleaved peptides of the APO-1 protein were subjected to amino acid sequencing, and corresponding oligonucleotides were used to identify a full-length APO-1 cDNA clone from an SKW6.4 cDNA library. The deduced amino acid sequence of APO-1 showed sequence identity with the Fas antigen, a cysteine-rich transmembrane protein of 335 amino acids with significant similarity to the members of the tumor necrosis factor/nerve growth factor receptor superfamily. The APO-1 antigen was expressed upon transfection of APO-1 cDNA into BL60-P7 Burkitt's lymphoma cells and conferred sensitivity towards anti-APO-1-induced apoptosis to the transfectants.
Disciplines :
Biochemistry, biophysics & molecular biology
Identifiers :
UNILU:UL-ARTICLE-2008-749
Author, co-author :
Oehm, A.
Behrmann, Iris ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Falk, W.
Pawlita, M.
Maier, G.
Klas, C.
Li-Weber, M.
Richards, S.
Dhein, J.
Trauth, B. C.
Language :
English
Title :
Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen
Publication date :
1992
Journal title :
Journal of Biological Chemistry
ISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology, Baltimore, United States - Maryland
Volume :
267
Issue :
15
Pages :
10709-15
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 12 September 2013

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