An antigen microarray protocol for COVID-19 serological analysis.

Antibody; Bioinformatics; Health Sciences; High Throughput Screening; Immunology; Proteomics; Antibodies, Viral; Antigens, Viral; Spike Glycoprotein, Coronavirus; Antibodies, Viral/blood; Antibodies, Viral/immunology; Antigens, Viral/blood; Antigens, Viral/immunology; COVID-19/blood; COVID-19/immunology; COVID-19/virology; Humans; Microarray Analysis/methods; SARS-CoV-2/immunology; Spike Glycoprotein, Coronavirus/blood; Spike Glycoprotein, Coronavirus/immunology; COVID-19; Microarray Analysis; SARS-CoV-2; Biochemistry, Genetics and Molecular Biology (all); Neuroscience (all); Immunology and Microbiology (all); Medicine (all); General Immunology and Microbiology; General Biochemistry, Genetics and Molecular Biology; General Neuroscience

Abstract :

[en] The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.

Disciplines :

Immunology & infectious disease

Author, co-author :

LONGWORTH, Joseph ; Luxembourg Institute of Health, RPPA Platform, 1A-B, rue Thomas Edison, 1445 Strassen, Luxembourg ; Proteomics of Cellular Signaling, Luxembourg of Health, 1A, Rue Thomas Edison, 1445 Strassen, Luxembourg

DITTMAR, Gunnar ; Proteomics of Cellular Signaling, Luxembourg of Health, 1A, Rue Thomas Edison, 1445 Strassen, Luxembourg

External co-authors :

Language :

English

Title :

An antigen microarray protocol for COVID-19 serological analysis.

Publication date :

17 September 2021

Journal title :

STAR Protocols

eISSN :

2666-1667

Publisher :

Cell Press, United States

Volume :

Issue :

Pages :

100815

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S2666166721005219?httpAccept=text/xml

Funders :

FNR

Funding text :

The COV-Array project is supported by the Luxembourg National Research Fund (FNR) (COV-Array, grant number 14879195 ) of the FNR (Luxembourg). We are thankful to the study participants, the Predi-COVID study group, the national ethics committee, and the funders for their support in the set-up of this initiative. The Predi-COVID study is supported by the Luxembourg National Research Fund (FNR) (Predi-COVID, grant number 14716273 ) and the André Losch Fondation . We thank Professor David C. James and Dr. Stephen R. P. Jaffé for kind provision of S1 protein produced in CHO cells. We would like to thank Dr. Christiane Hilger for discussion. We thank Dr. Daniel Perez Hernandez and Dr. Marta Mendes for testing the R application and providing feedback.

Available on ORBilu :

since 29 November 2023

Statistics

Number of views

7 (5 by Unilu)

Number of downloads

1 (0 by Unilu)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

WoS citations^™

Bibliography

de Assis, R.R., Jain, A., Nakajima, R., Jasinskas, A., Felgner, J., Obiero, J.M., Norris, P.J., Stone, M., Simmons, G., Bagri, A., et al. Analysis of SARS-CoV-2 antibodies in COVID-19 convalescent blood using a coronavirus antigen microarray. Nat. Commun., 12, 2021, 10.1038/s41467-020-20095-2.
Benton, D.J., Wrobel, A.G., Roustan, C., Borg, A., Xu, P., Martin, S.R., Rosenthal, P.B., Skehel, J.J., Gamblin, S.J., The effect of the D614G substitution on the structure of the spike glycoprotein of SARS-CoV-2. Proc. Natl. Acad. Sci. U S A, 118, 2021 e2022586118.
Burrell, C.J., Howard, C.R., Murphy, F.A., Coronaviruses. Fenner and White's Medical Virology, 2017, 437–446.
Coombes, K.R., Neeley, S., Joy, C., Hu, J., Baggerly, K., Roebuck, P., SuperCurve: RPPA analysis package. https://R-Forge.R-project.org/projects/supercurve/, 2017.
Fagherazzi, G., Fischer, A., Betsou, F., Vaillant, M., Ernens, I., Masi, S., Mossong, J., Staub, T., Brault, D., Bahlawane, C., et al. Protocol for a prospective, longitudinal cohort of people with COVID-19 and their household members to study factors associated with disease severity: the Predi-COVID study. BMJ Open, 10, 2020, e041834.
Jayaram, H., Fan, H., Bowman, B.R., Ooi, A., Jayaram, J., Collisson, E.W., Lescar, J., Prasad, B.V., X-Ray structures of the N- and C-Terminal domains of a coronavirus nucleocapsid protein: Implications for nucleocapsid formation. J. Virol. 80 (2006), 6612–6620.
Johari, Y.B., Jaffé, S.R.P., Scarrott, J.M., Johnson, A.O., Mozzanino, T., Pohle, T.H., Maisuria, S., Bhayat-Cammack, A., Lambiase, G., Brown, A.J., et al. Production of trimeric SARS-CoV-2 spike protein by CHO cells for serological COVID-19 testing. Biotechnol. Bioeng. 118 (2021), 1013–1021.
Mandala, V.S., McKay, M.J., Shcherbakov, A.A., Dregni, A.J., Kolocouris, A., Hong, M., Structure and drug binding of the SARS-CoV-2 envelope protein transmembrane domain in lipid bilayers. Nat. Struct. Mol. Biol. 27 (2020), 1202–1208.
Okba, N.M.A., Raj, V.S., Widjaja, I., GeurtsvanKessel, C.H., de Bruin, E., Chandler, F.D., Park, W.B., Kim, N.J., Farag, E.A.B.A., Al-Hajri, M., et al. Sensitive and specific detection of low-level antibody responses in mild middle east respiratory syndrome coronavirus infections. Emerging Infect. Dis. 25 (2019), 1868–1877.
Reusken, C., Mou, H., Godeke, G.J., Hoek, L. van der, Meyer, B., Müller, M.A., Haagmans, B., de Sousa, R., Schuurman, N., Dittmer, U., et al. Specific serology for emerging human coronaviruses by protein microarray. Eurosurveillance, 18, 2013, 20441 European Centre for Disease Prevention and Control.