Reference : Molecular basis for cellular effects of naturally occurring polyamines
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Molecular basis for cellular effects of naturally occurring polyamines
Bueb, Jean-Luc mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Mousli, M. [> >]
Landry, Y. [> >]
Agents and Actions
Birkhauser Verlag
Yes (verified by ORBilu)
[en] cytology ; Pertussis Toxin ; Peritoneal Cavity ; Mast Cells ; Male ; Histamine Release ; GTP-Binding Proteins ; GTP Phosphohydrolases ; Cadaverine ; Polyamines ; Putrescine ; Rats ; physiology ; drug effects ; metabolism ; pharmacology ; Virulence Factors, Bordetella ; Spermine ; Spermidine ; Rats, Inbred Strains ; Animals
[en] The naturally occurring polyamines, putrescine, spermidine and spermine, and the analogue cadaverine, induce a dose-dependent histamine release from rat peritoneal mast cells. Spermine was the most active among these polycationic metabolites, followed by spermidine and putrescine. The histamine release was inhibited by a 2 h pretreatment of the cells with pertussis toxin (100 ng/ml), demonstrating the involvement of a pertussis toxin-sensitive GTP-binding regulatory protein during the exocytotic process. Experiments performed with purified Go/Gi proteins reconstituted into phospholipid vesicles showed a direct stimulation of GTPase activity by the polyamines. This direct stimulation of G proteins and the consequent activation of the coupled effectors may represent a new mechanism of action for natural polyamines controlling receptor-dependent processes.

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