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Article (Scientific journals)
Oxidative stress activates MMP-2 in cultured human coronary smooth muscle cells
Valentin, F.; Bueb, Jean-Luc; Kieffer, P. et al.
2005In Fundamental and Clinical Pharmacology, 19 (6), p. 661-7
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Keywords :
enzymology; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Enzyme Induction; Enzyme-Linked Immunosorbent Assay; Humans; Matrix Metalloproteinase 2; Myocardium; Oxidative Stress; biosynthesis; metabolism; Cells, Cultured
Abstract :
[en] Oxidative stress is a cardinal feature of the inflammatory process and is involved in various pathologies including atherosclerosis. One of the important mechanisms in which oxidative stress may play a role is activation of matrix metalloproteinases such as MMP-2, which are involved in plaque destabilization. We investigated the mechanisms by which oxidative stress induces MMP-2 activation in cultured human coronary artery smooth muscle cells. Using zymography and Western blot analysis, we showed that oxidized low-density lipoproteins activate MMP-2 through up-regulation of the expression and activation of a membrane-type 1 matrix metalloproteinase (MT1-MMP). A second mechanism of MMP-2 activation involves oxidative radicals generated by the xanthine/xanthine oxidase complex (X/Xo). Research on these two mechanisms of MMP activation could lead to the elaboration of new vascular therapies for the treatment of atheroma based on interruption of a specific oxidative stress pathway.
Disciplines :
Biochemistry, biophysics & molecular biology
Identifiers :
UNILU:UL-ARTICLE-2008-530
Author, co-author :
Valentin, F.
Bueb, Jean-Luc ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Kieffer, P.
Tschirhart, Eric ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Atkinson, J.
Language :
English
Title :
Oxidative stress activates MMP-2 in cultured human coronary smooth muscle cells
Publication date :
2005
Journal title :
Fundamental and Clinical Pharmacology
ISSN :
1472-8206
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
19
Issue :
6
Pages :
661-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 09 September 2013

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