[en] The erythrocyte (or red blood cell) sedimentation rate (ESR) is commonly interpreted as a measure of cell aggregation and as a biomarker of inflammation. It is well known that an increase of fibrinogen concentration, an aggregation-inducing protein for erythrocytes, leads to an increase of the sedimentation rate of erythrocytes, which is generally explained through the formation and faster settling of large disjoint aggregates. However, many aspects of erythrocyte sedimentation conform well with the collapse of a particle gel rather than with the sedimentation of disjoint aggregates. Using experiments and cell-level numerical simulations, we systematically investigate the dependence of ESR on fibrinogen concentration and its relation to the microstructure of the gel-like erythrocyte suspension. We show that for physiological aggregation interactions, an increase in the attraction strength between cells results in a cell network with larger void spaces. This geometrical change in the network structure occurs due to anisotropic shape and deformability of erythrocytes and leads to an increased gel permeability and faster sedimentation. Our results provide a comprehensive relation between the ESR and the cell-level structure of erythrocyte suspensions and support the gel hypothesis in the interpretation of blood sedimentation.
Disciplines :
Physics
Author, co-author :
Dasanna, Anil Kumar; Theoretical Physics of Living Matter, Institute of Biological Information Processing and Institute for Advanced Simulation, Forschungszentrum Jülich, 52425 Jülich, Germany
Gerhard, Gompper; Theoretical Physics of Living Matter, Institute of Biological Information Processing and Institute for Advanced Simulation, Forschungszentrum Jülich, 52425 Jülich, Germany
Kaestner, Lars; Experimental Physics, Saarland University, 66123 Saarbruecken, Germany ; Theoretical Medicine and Biosciences, Saarland University, 66424 Homburg, Germany
WAGNER, Christian ; University of Luxembourg > Department of Physics and Materials Science
Fedosov, Dmitry A.; Theoretical Physics of Living Matter, Institute of Biological Information Processing and Institute for Advanced Simulation, Forschungszentrum Jülich, 52425 Jülich, Germany
External co-authors :
yes
Title :
Erythrocyte sedimentation: Effect of aggregation energy on gel structure during collapse
Publication date :
2022
Journal title :
Physical Review. E
ISSN :
2470-0045
eISSN :
2470-0053
Publisher :
AMER PHYSICAL SOC, ONE PHYSICS ELLIPSE, COLLEGE PK, MD 20740-3844 USA, Unknown/unspecified
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See Supplemental Material at http://link.aps.org/supplemental/10.1103/PhysRevE.105.024610 for Supplemental Materials. They contain a short discussion of the influence of cell rigidity on the delay time, a discussion of the influence of aggregation energy on the percolation, Supplemental Fig. 3 (illustration of pore sizes determination by numerical simulations), Supplemental Fig. 4 (hole size PDF from simulations), Supplemental Fig. 5 (hole size PDF), Supplemental Fig. 6 (structures obtained in various conditions), and Supplemental Movie S1/H40ImageJ.avi (illustrative movie generated from numerical simulations).
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