A Metabolomics-Based Investigation of the Effects of a Short-Term Body Weight Reduction Program in a Cohort of Adolescents with Obesity: A Prospective Interventional Clinical Study
[en] Metabolomics applied to assess the response to a body weight reduction program (BWRP) may generate valuable information concerning the biochemical mechanisms/pathways underlying the BWRP-induced cardiometabolic benefits. The aim of the present study was to establish the BWRP-induced changes in the metabolomic profile that characterizes the obese condition. In particular, a validated liquid chromatography–tandem mass spectrometry (LC–MS/MS) targeted metabolomic approach was used to determine a total of 188 endogenous metabolites in the plasma samples of a cohort of 42 adolescents with obesity (female/male = 32/10; age = 15.94 ± 1.33 year; body mass index standard deviation score (BMI SDS) = 2.96 ± 0.46) who underwent a 3-week BWRP, including hypocaloric diet, physical exercise, nutritional education, and psychological support. The BWRP was capable of significantly improving body composition (e.g., BMI SDS, p < 0.0001), glucometabolic homeostasis (e.g., glucose, p < 0.0001), and cardiovascular function (e.g., diastolic blood pressure, p = 0.016). A total of 64 metabolites were significantly reduced after the intervention (at least p < 0.05), including 53 glycerophospholipids (23 PCs ae, 21 PCs aa, and 9 lysoPCs), 7 amino acids (tyrosine, phenylalanine, arginine, citrulline, tryptophan, glutamic acid, and leucine), the biogenic amine kynurenine, 2 sphingomyelins, and (free) carnitine (C0). On the contrary, three metabolites were significantly increased after the intervention (at least p < 0.05)—in particular, glutamine, trans-4-hydroxyproline, and the octadecenoyl-carnitine (C18:1). In conclusion, when administered to adolescents with obesity, a short-term BWRP is capable of changing the metabolomic profile in the plasma.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Rigamonti, Antonello E. ✱; University of Milan > Department of Clinical Sciences and Community Health
FRIGERIO, Gianfranco ✱; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Environmental Cheminformatics
Caroli, Diana; Istituto Auxologico Italiano, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) > Experimental Laboratory for Auxo-Endocrinological Research
De Col, Alessandra; Istituto Auxologico Italiano, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) > Experimental Laboratory for Auxo-Endocrinological Research
Cella, Silvano G.; University of Milan > Department of Clinical Sciences and Community Health
Sartorio, Alessandro; Istituto Auxologico Italiano, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) > Experimental Laboratory for Auxo-Endocrinological Research
Fustinoni, Silvia; University of Milan > Department of Clinical Sciences and Community Health
✱ These authors have contributed equally to this work.
External co-authors :
yes
Language :
English
Title :
A Metabolomics-Based Investigation of the Effects of a Short-Term Body Weight Reduction Program in a Cohort of Adolescents with Obesity: A Prospective Interventional Clinical Study
Publication date :
19 January 2023
Journal title :
Nutrients
ISSN :
2072-6643
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Basel, Switzerland
The research was funded by the Italian Ministry of Health. The two metabolomics kits used were purchased in the frame of the grant “Call Piattaforme 2018” by Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico. G.F. is currently supported by the Luxembourg National Research Fund (FNR) (project: A18/BM/12341006).
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