RvD1(n-3 DPA) Downregulates the Transcription of Pro-Inflammatory Genes in Oral Epithelial Cells and Reverses Nuclear Translocation of Transcription Factor p65 after TNF-α Stimulation.

Balta, Maria G.; Schreurs, Olav; Halder, Rashi; Küntziger, Thomas M.; Saetre, Frank; Blix, Inger Johanne S.; Baekkevold, Espen S.; Glaab, Enrico; Schenck, Karl

doi:10.3390/ijms232314878

Reference : RvD1(n-3 DPA) Downregulates the Transcription of Pro-Inflammatory Genes in Oral Epith...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Multidisciplinary, general & others Human health sciences : Multidisciplinary, general & others
	Focus Areas :	Systems Biomedicine
	To cite this reference:	http://hdl.handle.net/10993/53432

Title :	RvD1(n-3 DPA) Downregulates the Transcription of Pro-Inflammatory Genes in Oral Epithelial Cells and Reverses Nuclear Translocation of Transcription Factor p65 after TNF-α Stimulation.
Language :	English
Author, co-author :	Balta, Maria G. [> >]
	Schreurs, Olav [> >]
	Halder, Rashi [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Scientific Central Services]
	Küntziger, Thomas M. [> >]
	Saetre, Frank [> >]
	Blix, Inger Johanne S. [> >]
	Baekkevold, Espen S. [> >]
	Glaab, Enrico [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Biomedical Data Science]
	Schenck, Karl [> >]
Publication date :	2022
Journal title :	International journal of molecular sciences
Volume :	23
Issue/season :	23
Peer reviewed :	Yes
Audience :	International
ISSN :	1422-0067
e-ISSN :	1422-0067
Country :	Switzerland
Keywords :	[en] Humans ; Transcription Factor RelA/genetics/metabolism ; Tumor Necrosis Factor-alpha/metabolism ; NF-kappa B/metabolism ; Active Transport, Cell Nucleus ; Inflammation/genetics/metabolism ; Epithelial Cells/metabolism ; NF-κB ; gingival ; oral epithelium ; oral inflammation ; p65 ; periodontitis ; resolvin ; specialized pro-resolving mediators
Abstract :	[en] Specialized pro-resolving mediators (SPMs) are multifunctional lipid mediators that participate in the resolution of inflammation. We have recently described that oral epithelial cells (OECs) express receptors of the SPM resolvin RvD1(n-3 DPA) and that cultured OECs respond to RvD1(n-3 DPA) addition by intracellular calcium release, nuclear receptor translocation and transcription of genes coding for antimicrobial peptides. The aim of the present study was to assess the functional outcome of RvD1(n-3 DPA)-signaling in OECs under inflammatory conditions. To this end, we performed transcriptomic analyses of TNF-α-stimulated cells that were subsequently treated with RvD1(n-3 DPA) and found significant downregulation of pro-inflammatory nuclear factor kappa B (NF-κB) target genes. Further bioinformatics analyses showed that RvD1(n-3 DPA) inhibited the expression of several genes involved in the NF-κB activation pathway. Confocal microscopy revealed that addition of RvD1(n-3 DPA) to OECs reversed TNF-α-induced nuclear translocation of NF-κB p65. Co-treatment of the cells with the exportin 1 inhibitor leptomycin B indicated that RvD1(n-3 DPA) increases nuclear export of p65. Taken together, our observations suggest that SPMs also have the potential to be used as a therapeutic aid when inflammation is established.
Research centres :	Luxembourg Centre for Systems Biomedicine (LCSB): Biomedical Data Science (Glaab Group)
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/10993/53432
DOI :	10.3390/ijms232314878
Other URL :	https://doi.org/10.3390/ijms232314878
Mentions required by the publisher for OA :	The original article is available at: https://doi.org/10.3390/ijms232314878

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