Reference : Morphological principles of neuronal mitochondria
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/51818
Morphological principles of neuronal mitochondria
English
Mendelsohn, Rachel [> >]
Garcia, Guadalupe C. [> >]
Bartol, Thomas M. [> >]
Lee, Christopher T. [> >]
Khandelwal, Priya [> >]
Liu, Emily [> >]
Spencer, Donald J. [> >]
Husar, Adam [> >]
Bushong, Eric A. [> >]
Phan, Sebastien [> >]
Perkins, Guy [> >]
Ellisman, Mark H. [> >]
Skupin, Alexander mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Integrative Cell Signalling]
Sejnowski, Terrence J. [> >]
Rangamani, Padmini [> >]
2022
Journal of Comparative Neurology
John Wiley & Sons
530
6
886--902
Yes (verified by ORBilu)
0021-9967
1096-9861
Hoboken
NY
[en] EM tomography ; energetics ; mitochondria ; morphology ; neuronal
[en] In the highly dynamic metabolic landscape of a neuron, mitochondrial membrane architectures can provide critical insight into the unique energy balance of the cell. Current theoretical calculations of functional outputs like adenosine triphosphate and heat often represent mitochondria as idealized geometries, and therefore, can miscalculate the metabolic fluxes. To analyze mitochondrial morphology in neurons of mouse cerebellum neuropil, 3D tracings of complete synaptic and axonal mitochondria were constructed using a database of serial transmission electron microscopy (TEM) tomography images and converted to watertight meshes with minimal distortion of the original microscopy volumes with a granularity of 1.64 nanometer isotropic voxels. The resulting in-silico representations were subsequently quantified by differential geometry methods in terms of the mean and Gaussian curvatures, surface areas, volumes, and membrane motifs, all of which can alter the metabolic output of the organelle. Finally, we identify structural motifs present across this population of mitochondria, which may contribute to future modeling studies of mitochondrial physiology and metabolism in neurons.
http://hdl.handle.net/10993/51818
10.1002/cne.25254
https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.25254
\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/cne.25254

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