Partial amplification of the measles virus nucleocapsid gene from stored sera and cerebrospinal fluids for molecular epidemiological studies

Kreis, Stephanie; Schoub, B. D.

doi:10.1002/(SICI)1096-9071(199810)56:2<174::AID-JMV12>3.0.CO;2-G

Reference : Partial amplification of the measles virus nucleocapsid gene from stored sera and cer...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/10993/4984

Title :	Partial amplification of the measles virus nucleocapsid gene from stored sera and cerebrospinal fluids for molecular epidemiological studies
Language :	English
Author, co-author :	Kreis, Stephanie [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
	Schoub, B. D. [> >]
Publication date :	1998
Journal title :	Journal of Medical Virology
Publisher :	Wiley Liss, Inc.
Volume :	56
Issue/season :	2
Pages :	174-177
Peer reviewed :	Yes (verified by ORBi^lu)
Audience :	International
ISSN :	0146-6615
e-ISSN :	1096-9071
City :	New York
Country :	NY
Keywords :	[en] Measles virus; Sequence analysis; Serum-RT-PCR
Abstract :	[en] The analysis of stored sera for retrospective molecular epidemiological studies provides a powerful tool to investigate strain variation in measles viruses that had circulated up to 20 years ago. For this purpose, a rapid and simple method for extraction of RNA from stored sera and cerebrospinal fluids (CSF) was developed. When used on sera and CSFs that have been frozen for as long as 20 years, this method proved to be more efficient than established techniques. The extracted RNA was reverse transcribed into cDNA by using random hexamer primers. The PCR amplification of the 3' terminus of the nucleocapsid gene (N) was divided into two overlapping fragments of 375 and 384 bp length, covering the entire region of interest. This region is thought to have the highest variability within the MV genome and has previously been shown to be suitable for strain characterization. The resulting PCR fragments were sequenced manually by using standard methods without the need of further clean-up steps.
Permalink :	http://hdl.handle.net/10993/4984
DOI :	10.1002/(SICI)1096-9071(199810)56:2<174::AID-JMV12>3.0.CO;2-G
Other URL :	http://www.scopus.com/inward/record.url?eid=2-s2.0-0031656503&partnerID=40&md5=2259de033bd836e61082b73dc5367f90
Commentary :	cited By (since 1996)8 Scopus

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