Reference : Distribution pattern of small RNA and degradome reads provides information on miRNA g...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Distribution pattern of small RNA and degradome reads provides information on miRNA gene structure and regulation.
Branscheid, Anja* [> >]
Devers, Emanuel A.* [> >]
May, Patrick* mailto [Max Planck Institute for Molecular Plant Physiology > Bioinformatics]
Krajinski, Franziska [> >]
* These authors have contributed equally to this work.
Plant Signaling and Behavior
Yes (verified by ORBilu)
United States
[en] Base Sequence ; Gene Expression Regulation, Plant ; Genetic Loci/genetics ; Genome, Plant/genetics ; Medicago truncatula/genetics/microbiology ; MicroRNAs/genetics ; Molecular Sequence Data ; Mycorrhizae/physiology ; RNA Stability/genetics ; RNA, Plant/genetics/metabolism
[en] Plant microRNAs (miRNAs) have an impact in the regulation of several biological processes such as development, growth and metabolism by negatively controlling gene expression at the post-transcriptional level. However, the role of these small molecules in the symbiotic interaction of plant roots and arbuscular mycorrhizal (AM) fungi remained elusive. To elucidate the role of miRNAs during AM symbiosis we used a deep sequencing approach to analyze the small RNA and degradome sequence tags of Medicago truncatula non-mycorrhizal and mycorrhizal roots. We identified 243 novel Medicago microRNAs and 118 mRNA cleavage targets of miRNA mature and star sequences. Several AM symbiosis-relevant genes were identified as miRNA targets. The transcript of MtNsp2, encoding a GRAS transcription factor involved in the nodule and mycorrhizal signaling pathway, is cleaved by a novel member of the miR171 gene family, namely miR171h. Here, we carried out a detailed analysis of the genomic structure of the MIR171h gene comprising our deep sequencing data. The results suggest a feedback circuit between mature miR171h and its own primary transcript showing the ability of this miRNA regulating itself.

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