Reference : In Vitro Red Blood Cell Segregation in Sickle Cell Anemia
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Physical, chemical, mathematical & earth Sciences : Multidisciplinary, general & others
In Vitro Red Blood Cell Segregation in Sickle Cell Anemia
Claveria, Viviana [Abkarian, M (Corresponding Author), Univ Montpellier, Ctr Biol Struct, CNRS UMR 5048, INSERM UMR 1054, Montpellier, France. Abkarian, M (Corresponding Author), Lab Excellence Globule Rouge Labex GR Ex, Paris, France. Claveria, Viviana]
Connes, Philippe [Lanotte, Luca]
Lanotte, Luca [Abkarian, Manouk, Univ Montpellier, Ctr Biol Struct, CNRS UMR 5048, INSERM UMR 1054, Montpellier, France. Claveria, Viviana]
Celine, Renoux [Wagner, Christian, Saarland Univ, Expt Phys, Saarbrucken, Germany. Connes, Philippe]
Joly, Philippe [Renoux, Celine]
Fort, Romain [Joly, Philippe]
Gauthier, Alexandra [Fort, Romain]
Wagner, Christian mailto [University of Luxembourg > Department of Physics and Material Science]
Abkarian, Manouk [Renoux, Celine]
??? [Joly, Philippe]
??? [Fort, Romain]
??? [Gauthier, Alexandra]
??? [Abkarian, Manouk, Lab Excellence Globule Rouge Labex GR Ex, Paris, France. Connes, Philippe, Inst Univ France, Paris, France. Renoux, Celine]
??? [Joly, Philippe, Hosp Civils Lyon, Ctr Biol Pathol Est, Lab Biochim Biol Mol, UF Biochim Pathol Erythrocytaires, Lyon, France. Fort, Romain, Hosp Civils Lyon, Hop Edouard Herriot, Med Interne, Lyon, France. Gauthier, Alexandra, Hosp Civils Lyon, IHOP, Lyon, France.]
[en] sickle cell anemia; red blood cells; segregation; red blood cell density; microfluidics MULTICOMPONENT SUSPENSIONS; VASCULAR ENDOTHELIUM; FREE LAYER; FLOW MARGINATION; SHAPE; HEMOGLOBIN; PARTICLES; ADHESION; DENSITY Physics Physics ; Multidisciplinary Labex Numev [ANR-10-LABX-20]; Idex MUSE [ANR-16-IDEX-0006] We would like to thank Labex Numev (Convention Grant No. ANR-10-LABX-20 Rheoblood project) as well as the Idex MUSE (Convention Grant No. ANR-16-IDEX-0006 ; Globule project). 47 0 0 0 Front. Physics XP3CR Science Citation Index Expanded (SCI-EXPANDED) WOS:000730747300001
[en] Red blood cells in sickle cell anemia (sRBC) are more heterogeneous in their physical properties than healthy red blood cells, spanning adhesiveness, rigidity, density, size, and shape. sRBC with increased adhesiveness to the vascular wall would trigger vaso-occlusive like complications, a hallmark of sickle cell anemia. We investigated whether segregation occurs among sRBC flowing in micron-sized channels and tested the impact of aggregation on segregation. Two populations of sRBC of different densities were separated, labeled, and mixed again. The mixed suspension was flowed within glass capillary tubes at different pressure-drops, hematocrit, and suspending media that promoted or not cell aggregation. Observations were made at a fixed channel position. The mean flow velocity was obtained by using the cells as tracking particles, and the cell depleted layer (CDL) by measuring the distance from the cell core border to the channel wall. The labeled sRBC were identified by stopping the flow and scanning the cells within the channel section. The tube hematocrit was estimated from the number of fluorescence cells identified in the field of view. In non-aggregating media, our results showed a heterogeneous distribution of sRBC according to their density: low-density sRBC population remained closer to the center of the channel, while the densest cells segregated towards the walls. There was no impact of the mean flow velocity and little impact of hematocrit. This segregation heterogeneity could influence the ability of sRBC to adhere to the vascular wall and slow down blood flow. However, promoting aggregation inhibited segregation while CDL thickness was enhanced by aggregation, highlighting a potential protective role against vaso-occlusion in patients with sickle cell anemia.

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