Article (Scientific journals)
Spatial Fluctuations at Vertices of Epithelial Layers: Quantification of Regulation by Rho Pathway
Fodor, Etienne; Mehandia, Vishwajeet; Comelles, Jordi et al.
2018In BIOPHYSICAL JOURNAL, 114 (4), p. 939-946
Peer reviewed
 

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Abstract :
[en] In living matter, shape fluctuations induced by acto-myosin are usually studied in vitro via reconstituted gels, whose properties are controlled by changing the concentrations of actin, myosin, and cross-linkers. Such an approach deliberately avoids consideration of the complexity of biochemical signaling inherent to living systems. Acto-myosin activity inside living cells is mainly regulated by the Rho signaling pathway which is composed of multiple layers of coupled activators and inhibitors. Here, we investigate how such a pathway controls the dynamics of confluent epithelial tissues by tracking the displacements of the junction points between cells. Using a phenomenological model to analyze the vertex fluctuations, we rationalize the effects of different Rho signaling targets on the emergent tissue activity by quantifying the effective diffusion coefficient, and the persistence time and length of the fluctuations. Our results reveal an unanticipated correlation between layers of activation/inhibition and spatial fluctuations within tissues. Overall, this work connects regulation via biochemical signaling with mesoscopic spatial fluctuations, with potential application to the study of structural rearrangements in epithelial tissues.
Disciplines :
Physics
Author, co-author :
Fodor, Etienne ;  University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Physics and Materials Science (DPHYMS)
Mehandia, Vishwajeet;  Laboratory of Cell Physics, ISIS/IGBMC, Université de Strasbourg and CNRS (UMR 7006), Strasbourg, France ; Development and Stem Cells Program, IGBMC, CNRS (UMR 7104), INSERM (U964), Université de Strasbourg, Illkirch, France ; School of Mechanical, Materials and Energy Engineering, Indian Institute of Technology, Ropar, India
Comelles, Jordi;  Laboratory of Cell Physics, ISIS/IGBMC, Université de Strasbourg and CNRS (UMR 7006), Strasbourg, France ; Development and Stem Cells Program, IGBMC, CNRS (UMR 7104), INSERM (U964), Université de Strasbourg, Illkirch, France
Thiagarajan, Raghavan;  Laboratory of Cell Physics, ISIS/IGBMC, Université de Strasbourg and CNRS (UMR 7006), Strasbourg, France ; Development and Stem Cells Program, IGBMC, CNRS (UMR 7104), INSERM (U964), Université de Strasbourg, Illkirch, France
Gov, Nir S.;  Department of Chemical Physics, Weizmann Institute of Science, Rehovot, Israel
Visco, Paolo;  Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS/P7, Université Paris Diderot, Paris cedex 13, France
Frederic, Van Wijland;  Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS/P7, Université Paris Diderot, Paris cedex 13, France
Riveline, Daniel;  Laboratory of Cell Physics, ISIS/IGBMC, Université de Strasbourg and CNRS (UMR 7006), Strasbourg, France ; Development and Stem Cells Program, IGBMC, CNRS (UMR 7104), INSERM (U964), Université de Strasbourg, Illkirch, France
External co-authors :
yes
Language :
English
Title :
Spatial Fluctuations at Vertices of Epithelial Layers: Quantification of Regulation by Rho Pathway
Publication date :
2018
Journal title :
BIOPHYSICAL JOURNAL
ISSN :
0006-3495
Publisher :
CELL PRESS, 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA, Unknown/unspecified
Volume :
114
Issue :
4
Pages :
939-946
Peer reviewed :
Peer reviewed
Focus Area :
Physics and Materials Science
Commentary :
Article
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