Reference : Spatial Fluctuations at Vertices of Epithelial Layers: Quantification of Regulation b...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
Physics and Materials Science
http://hdl.handle.net/10993/47919
Spatial Fluctuations at Vertices of Epithelial Layers: Quantification of Regulation by Rho Pathway
English
Fodor, Etienne mailto [University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Physics and Materials Science (DPHYMS)]
Mehandia, Vishwajeet [Laboratory of Cell Physics, ISIS/IGBMC, Université de Strasbourg and CNRS (UMR 7006), Strasbourg, France > > > ; Development and Stem Cells Program, IGBMC, CNRS (UMR 7104), INSERM (U964), Université de Strasbourg, Illkirch, France > > > ; School of Mechanical, Materials and Energy Engineering, Indian Institute of Technology, Ropar, India]
Comelles, Jordi [Laboratory of Cell Physics, ISIS/IGBMC, Université de Strasbourg and CNRS (UMR 7006), Strasbourg, France > > > ; Development and Stem Cells Program, IGBMC, CNRS (UMR 7104), INSERM (U964), Université de Strasbourg, Illkirch, France]
Thiagarajan, Raghavan [Laboratory of Cell Physics, ISIS/IGBMC, Université de Strasbourg and CNRS (UMR 7006), Strasbourg, France > > > ; Development and Stem Cells Program, IGBMC, CNRS (UMR 7104), INSERM (U964), Université de Strasbourg, Illkirch, France]
Gov, Nir S. [Department of Chemical Physics, Weizmann Institute of Science, Rehovot, Israel]
Visco, Paolo [Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS/P7, Université Paris Diderot, Paris cedex 13, France]
Frederic, Van Wijland [Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS/P7, Université Paris Diderot, Paris cedex 13, France]
Riveline, Daniel [Laboratory of Cell Physics, ISIS/IGBMC, Université de Strasbourg and CNRS (UMR 7006), Strasbourg, France > > > ; Development and Stem Cells Program, IGBMC, CNRS (UMR 7104), INSERM (U964), Université de Strasbourg, Illkirch, France]
2018
BIOPHYSICAL JOURNAL
CELL PRESS
114
4
939-946
Yes (verified by ORBilu)
International
0006-3495
50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA
[en] In living matter, shape fluctuations induced by acto-myosin are usually studied in vitro via reconstituted gels, whose properties are controlled by changing the concentrations of actin, myosin, and cross-linkers. Such an approach deliberately avoids consideration of the complexity of biochemical signaling inherent to living systems. Acto-myosin activity inside living cells is mainly regulated by the Rho signaling pathway which is composed of multiple layers of coupled activators and inhibitors. Here, we investigate how such a pathway controls the dynamics of confluent epithelial tissues by tracking the displacements of the junction points between cells. Using a phenomenological model to analyze the vertex fluctuations, we rationalize the effects of different Rho signaling targets on the emergent tissue activity by quantifying the effective diffusion coefficient, and the persistence time and length of the fluctuations. Our results reveal an unanticipated correlation between layers of activation/inhibition and spatial fluctuations within tissues. Overall, this work connects regulation via biochemical signaling with mesoscopic spatial fluctuations, with potential application to the study of structural rearrangements in epithelial tissues.
http://hdl.handle.net/10993/47919
10.1016/j.bpj.2017.12.026
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