Reference : Active Mechanics Reveal Molecular-Scale Force Kinetics in Living Oocytes
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
Physics and Materials Science
http://hdl.handle.net/10993/47918
Active Mechanics Reveal Molecular-Scale Force Kinetics in Living Oocytes
English
Ahmed, Wylie W. [Department of Physics, California State University, Fullerton, California > > > ; Laboratoire Physico-Chimie Curie, Institut Curie, PSL Research University, Paris, France > > > ; Sorbonne Universités, UPMC Université Paris 06, Paris, France]
Fodor, Etienne mailto [University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Physics and Materials Science (DPHYMS)]
Almonacid, Maria [CIRB, Collège de France, and CNRS-UMR7241 and INSERM-U1050, Equipe Labellisée Fondation pour la Recherche Médicale, Paris, France]
Matthias, Bussonnier [Laboratoire Physico-Chimie Curie, Institut Curie, PSL Research University, Paris, France > > > ; Sorbonne Universités, UPMC Université Paris 06, Paris, France]
Verlhac, Marie-Helene [CIRB, Collège de France, and CNRS-UMR7241 and INSERM-U1050, Equipe Labellisée Fondation pour la Recherche Médicale, Paris, France]
Gov, Nir [Department of Chemical Physics, Weizmann Institute of Science, Rehovot, Israel]
Visco, Paolo [Laboratoire Matière et Systèmes Complexes, Université Paris Diderot, Paris, France]
van Wijland, Frederic [Laboratoire Matière et Systèmes Complexes, Université Paris Diderot, Paris, France]
Betz, Timo [Laboratoire Physico-Chimie Curie, Institut Curie, PSL Research University, Paris, France > > > ; Sorbonne Universités, UPMC Université Paris 06, Paris, France > > > ; Institute of Cell Biology, Center for Molecular Biology of Inflammation, Cells-in-Motion Cluster of Excellence, Münster University, Münster, Germany]
2018
BIOPHYSICAL JOURNAL
CELL PRESS
114
7
1667-1679
Yes (verified by ORBilu)
International
0006-3495
50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA
[en] Active diffusion of intracellular components is emerging as an important process in cell biology. This process is mediated by complex assemblies of molecular motors and cytoskeletal filaments that drive force generation in the cytoplasm and facilitate enhanced motion. The kinetics of molecular motors have been precisely characterized in vitro by single molecule approaches, but their in vivo behavior remains elusive. Here we study the active diffusion of vesicles in mouse oocytes, where this process plays a key role in nuclear positioning during development, and combine an experimental and theoretical framework to extract molecular-scale force kinetics (force, power stroke, and velocity) of the in vivo active process. Assuming a single dominant process, we find that the nonequilibrium activity induces rapid kicks of duration tau similar to 300 mu s resulting in an average force of F similar to 0.4 pN on vesicles in in vivo oocytes, remarkably similar to the kinetics of in vitro myosin-V. Our results reveal that measuring in vivo active fluctuations allows extraction of the molecular-scale activity in agreement with single-molecule studies and demonstrates a mesoscopic framework to access force kinetics.
http://hdl.handle.net/10993/47918
10.1016/j.bpj.2018.02.009
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