Reference : Effect of Red Blood Cell Aging In Vivo on Their Aggregation Properties In Vitro: Meas...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
Physical, chemical, mathematical & earth Sciences : Physics
Engineering, computing & technology : Materials science & engineering
Engineering, computing & technology : Multidisciplinary, general & others
http://hdl.handle.net/10993/45605
Effect of Red Blood Cell Aging In Vivo on Their Aggregation Properties In Vitro: Measurements with Laser Tweezers
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Ermolinskiy, Petr [Lomonosov Moscow State Univ, Fac Phys, Moscow 119991, Russia.]
Lugovtsov, Andrei [Lomonosov Moscow State Univ, Fac Phys, Moscow 119991, Russia.]
Yaya, Francois [Saarland Univ, Expt Phys, D-66123 Saarbrucken, Germany.]
Lee, Kisung [Inst Basic Sci, Ctr Soft & Living Matter, Ulsan 44919, South Korea.]
Kaestner, Lars [Saarland Univ, Expt Phys, D-66123 Saarbrucken, Germany.]
Wagner, Christian mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit]
Priezzhev, Alexander [Lomonosov Moscow State Univ, Fac Phys, Moscow 119991, Russia.]
2020
Applied Sciences
Mdpi
10
21
7581-10
Yes (verified by ORBilu)
2076-3417
Basel
[en] red blood cells ; optical tweezers ; RBC aggregation ; RBC aging ; aggregation force ; Percoll
[en] Red blood cell (RBC) aggregation highly influences hemorheology and blood microcirculation in the human body. The aggregation properties of RBCs can vary due to numerous factors, including RBC age. The aim of this work was to estimate in vitro the differences in the RBC aggregation properties of different RBC age populations in single-cell experiments using laser tweezers. RBCs from five healthy volunteers were separated into four subpopulations by Percoll density gradient centrifugation. Each subpopulation of the RBC was separately resuspended in autologous plasma or dextran 70 kDa (50 mg/mL). The aggregation force between the single cells was measured with holographic laser tweezers. The obtained data demonstrated an enhancement of RBC aggregation force in doublets with age: the older the cells, the higher the aggregation force. The obtained data revealed the differences between the aggregation and aggregability of RBC in dependence of the RBC in vivo age.
Russian Science FoundationRussian Science Foundation (RSF) [18-15-00422] ; French German University (DFH/UFA)
http://hdl.handle.net/10993/45605
10.3390/app10217581
This work was supported by the Russian Science Foundation grant No 18-15-00422. F.Y. and C.W. acknowledge funding from the French German University (DFH/UFA).

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