Article (Scientific journals)
Effect of Red Blood Cell Aging In Vivo on Their Aggregation Properties In Vitro: Measurements with Laser Tweezers
Ermolinskiy, Petr; Lugovtsov, Andrei; Yaya, Francois et al.
2020In Applied Sciences, 10 (21), p. 7581-10
Peer Reviewed verified by ORBi
 

Files


Full Text
2020RBC-aging_AppliedScience.pdf
Publisher postprint (2.56 MB)
Download

All documents in ORBilu are protected by a user license.

Send to



Details



Keywords :
red blood cells; optical tweezers; RBC aggregation; RBC aging; aggregation force; Percoll
Abstract :
[en] Red blood cell (RBC) aggregation highly influences hemorheology and blood microcirculation in the human body. The aggregation properties of RBCs can vary due to numerous factors, including RBC age. The aim of this work was to estimate in vitro the differences in the RBC aggregation properties of different RBC age populations in single-cell experiments using laser tweezers. RBCs from five healthy volunteers were separated into four subpopulations by Percoll density gradient centrifugation. Each subpopulation of the RBC was separately resuspended in autologous plasma or dextran 70 kDa (50 mg/mL). The aggregation force between the single cells was measured with holographic laser tweezers. The obtained data demonstrated an enhancement of RBC aggregation force in doublets with age: the older the cells, the higher the aggregation force. The obtained data revealed the differences between the aggregation and aggregability of RBC in dependence of the RBC in vivo age.
Disciplines :
Chemistry
Physics
Materials science & engineering
Engineering, computing & technology: Multidisciplinary, general & others
Author, co-author :
Ermolinskiy, Petr;  Lomonosov Moscow State Univ, Fac Phys, Moscow 119991, Russia.
Lugovtsov, Andrei;  Lomonosov Moscow State Univ, Fac Phys, Moscow 119991, Russia.
Yaya, Francois;  Saarland Univ, Expt Phys, D-66123 Saarbrucken, Germany.
Lee, Kisung;  Inst Basic Sci, Ctr Soft & Living Matter, Ulsan 44919, South Korea.
Kaestner, Lars;  Saarland Univ, Expt Phys, D-66123 Saarbrucken, Germany.
Wagner, Christian ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit
Priezzhev, Alexander;  Lomonosov Moscow State Univ, Fac Phys, Moscow 119991, Russia.
External co-authors :
yes
Title :
Effect of Red Blood Cell Aging In Vivo on Their Aggregation Properties In Vitro: Measurements with Laser Tweezers
Publication date :
2020
Journal title :
Applied Sciences
ISSN :
2076-3417
Publisher :
Mdpi, Basel, Unknown/unspecified
Volume :
10
Issue :
21
Pages :
7581-10
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Russian Science FoundationRussian Science Foundation (RSF) [18-15-00422]
French German University (DFH/UFA)
Commentary :
This work was supported by the Russian Science Foundation grant No 18-15-00422. F.Y. and C.W. acknowledge funding from the French German University (DFH/UFA).
Available on ORBilu :
since 18 January 2021

Statistics


Number of views
60 (4 by Unilu)
Number of downloads
55 (0 by Unilu)

Scopus citations®
 
15
Scopus citations®
without self-citations
9
OpenCitations
 
8
WoS citations
 
14

Bibliography


Similar publications



Contact ORBilu