Reference : Förster-resonance energy transfer between diffusing molecules and a functionalized pl...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
Physics and Materials Science
http://hdl.handle.net/10993/44591
Förster-resonance energy transfer between diffusing molecules and a functionalized plasmonic nanopore
English
Zambrana-Puyalto, Xavier []
Ponzellini, Paolo []
Maccaferri, Nicolò mailto [University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Physics and Materials Science (DPHYMS) >]
Garoli, Denis []
2020
Physical Review Applied
American Physical Society
14
5
054065
Yes (verified by ORBilu)
International
2331-7019
2331-7043
College Park
MD
[en] Plasmonic nanopores are the subject of extensive investigations as a potential platform to enable efficient optical read-out in translocation experiments with biomolecules such as DNA and proteins. They allow for the engineering of electromagnetic fields at the nanoscale, which are typically used to enhance the emission efficiency of fluorescent molecules. Their features make them suitable for detection strategies based on the energy transfer between translocating molecules and the nanopore itself. Here, we have carried out an optical experiment to show that a handful of diffusing dyes acting as donors can exchange energy via Fster resonance energy transfer (FRET) with a gold nanopore functionalized with dyes behaving as acceptors. The FRET pair is composed of ATTORho6G (donor) and Alexa610 (acceptor). To perform this proof-of-concept experiment, we used a gold nanopore with a diameter of 80 nm, prepared on a Si3N4 membrane. We have observed that the presence of the acceptors on the walls of the nanopore reduces the lifetime of the diffusing donors. In addition, we have observed that the presence of the acceptors reduces the fluorescence signal on the donor detection channel and increases the fluorescence signal on the acceptor detection channel. The combination of these three effects gives us enough evidence to claim that the diffusing donors exchange energy with the functionalized nanopore via FRET, despite the relatively large size of the nanopore. The FRET efficiency of the process has been found to be of the order of 30%, which is in a fairly good agreement with a theoretical value obtained using a simplified model.
http://hdl.handle.net/10993/44591
10.1103/PhysRevApplied.14.054065
https://journals.aps.org/prapplied/abstract/10.1103/PhysRevApplied.14.054065

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Limited access
2020_phy_rev_app.pdfAuthor preprint881.18 kBRequest a copy

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.