Reference : Dynamic insights on transcription initiation and RNA processing during bacterial adap...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/10993/42936
Dynamic insights on transcription initiation and RNA processing during bacterial adaptation
English
Lacoux, Caroline [> >]
Fouquier d'Hérouël, Aymeric mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Wessner- Le Bohec, Françoise [> >]
Innocenti, Nicolas [> >]
Bohn, Chantal [> >]
Kennedy, Sean P. [> >]
Rochat, Tatiana [> >]
Bonnin, Rémy [> >]
Serror, Pascale [> >]
Aurell, Erik [> >]
Bouloc, Philippe [> >]
Repoila, Francis [> >]
2020
RNA
Yes
International
[en] Transcription initiation and RNA processing govern gene expression and enable bacterial adaptation by reshaping the RNA landscape. The aim of this study was to simultaneously observe these two fundamental processes in a transcriptome responding to an environmental signal. A controlled σE system in E. coli was coupled to our previously-described tagRNA-seq method to yield process kinetics information. Changes in transcription initiation frequencies (TIF) and RNA processing frequencies (PF) were followed using 5’ RNA tags. Changes in TIF showed a binary increased/decreased pattern that alternated between transcriptionally activated and repressed promoters, providing the bacterial population with transcriptional oscillation. PF variation fell into three categories of cleavage activity; i) constant and independent of RNA levels, ii) increased once RNA has accumulated, and iii) positively correlated to changes in TIF. This work provides a comprehensive and dynamic view of major events leading to transcriptomic reshaping during bacterial adaptation. It unveils an interplay between transcription initiation and the activity of specific RNA cleavage sites. This study utilized a well-known genetic system to analyze fundamental processes, and can serve as blueprint for comprehensive studies that exploit the RNA metabolism to decipher and understand bacterial gene expression control.
Researchers
http://hdl.handle.net/10993/42936
10.1261/rna.073288.119
http://rnajournal.cshlp.org/content/early/2020/01/28/rna.073288.119.abstract

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Limited access
RNA-2020-Lacoux-rna.073288.119.pdfAuthor preprint899.98 kBRequest a copy

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.