Article (Scientific journals)
Microbiota alter metabolism and mediate neurodevelopmental toxicity of 17β-estradiol
Catron, Tara R.; Swank, Adam; Wehmas, Leah C. et al.
2019In Scientific Reports, 9 (1), p. 7064
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Abstract :
[en] Estrogenic chemicals are widespread environmental contaminants associated with diverse health and ecological effects. During early vertebrate development, estrogen receptor signaling is critical for many different physiologic responses, including nervous system function. Recently, host-associated microbiota have been shown to influence neurodevelopment. Here, we hypothesized that microbiota may biotransform exogenous 17-βestradiol (E2) and modify E2 effects on swimming behavior. Colonized zebrafish were continuously exposed to non-teratogenic E2 concentrations from 1 to 10 days post-fertilization (dpf). Changes in microbial composition and predicted metagenomic function were evaluated. Locomotor activity was assessed in colonized and axenic (microbe-free) zebrafish exposed to E2 using a standard light/dark behavioral assay. Zebrafish tissue was collected for chemistry analyses. While E2 exposure did not alter microbial composition or putative function, colonized E2-exposed larvae showed reduced locomotor activity in the light, in contrast to axenic E2-exposed larvae, which exhibited normal behavior. Measured E2 concentrations were significantly higher in axenic relative to colonized zebrafish. Integrated peak area for putative sulfonated and glucuronidated E2 metabolites showed a similar trend. These data demonstrate that E2 locomotor effects in the light phase are dependent on the presence of microbiota and suggest that microbiota influence chemical E2 toxicokinetics. More broadly, this work supports the concept that microbial colonization status may influence chemical toxicity.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Catron, Tara R.
Swank, Adam
Wehmas, Leah C.
Phelps, Drake
Keely, Scott P.
Brinkman, Nichole E.
McCord, James
Singh, Randolph ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Sobus, Jon
Wood, Charles E.
Strynar, Mark
Wheaton, Emily
Tal, Tamara
More authors (3 more) Less
External co-authors :
yes
Language :
English
Title :
Microbiota alter metabolism and mediate neurodevelopmental toxicity of 17β-estradiol
Publication date :
2019
Journal title :
Scientific Reports
ISSN :
2045-2322
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
9
Issue :
1
Pages :
7064
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 23 January 2020

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