Reference : Covalent functionalization of graphene oxide with D-mannose: evaluating the hemolytic...
Scientific journals : Article
Engineering, computing & technology : Chemical engineering
Systems Biomedicine
http://hdl.handle.net/10993/41878
Covalent functionalization of graphene oxide with D-mannose: evaluating the hemolytic effect and protein corona formation
English
Sousa, Marcelo De [University of Campinas > Laboratory of Solid State Chemistry]
Martins, Carlos H. Z. [University of Campinas > Laboratory of Solid State Chemistry]
Franqui, Lidiane S. [Brazilian Center for Research in Energy and Materials (CNPEM) > Brazilian Nanotechnology National Laboratory (LNNano) > > ; University of Campinas > School of Technology]
Fonseca, Leandro C. [University of Campinas > Laboratory of Solid State Chemistry]
Delite, Fabrício S. [Brazilian Center for Research in Energy and Materials (CNPEM) > Brazilian Nanotechnology National Laboratory (LNNano)]
Martin Lanzoni, Evandro mailto [Brazilian Center for Research in Energy and Materials (CNPEM) > Brazilian Nanotechnology National Laboratory (LNNano)]
Martinez, Diego Stéfani T. [University of Campinas > Laboratory of Solid State Chemistry > > ; Brazilian Center for Research in Energy and Materials (CNPEM) > Brazilian Nanotechnology National Laboratory (LNNano) > > ; University of Campinas > School of Technology]
Alves, Oswaldo L. [University of Campinas > Laboratory of Solid State Chemistry]
2018
Journal of Materials Chemistry B
6
18
2803--2812
Yes
2050-7518
[en] In this work, graphene oxide (GO) was covalently functionalized with D-mannose (man-GO) using mannosylated ethylenediamine. XPS (C1s and N1s) confirmed the functionalization of GO through the binding energies at 288.2 eV and 399.8 eV, respectively, which are attributed to the amide bond. ATR-FTIR spectroscopy showed an increase in the amine bond intensity, at 1625 cm−1 (stretching CO), after the functionalization step. Furthermore, the man-GO toxicity to human red blood cells (hemolysis) and its nanobiointeractions with human plasma proteins (hard corona formation) were evaluated. The mannosylation of GO drastically reduced its toxicity to red blood cells. SDS-PAGE analysis showed that the mannosylation process of GO also drastically reduced the amount of the proteins in the hard corona. Additionally, proteomics analysis by LC–MS/MS revealed 109 proteins in the composition of the man-GO hard corona. Finally, this work contributes to future biomedical applications of graphene-based materials functionalized with active biomolecules.
http://hdl.handle.net/10993/41878
10.1039/C7TB02997G
http://pubs.rsc.org/en/content/articlelanding/2018/tb/c7tb02997g
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