Reference : Intracellular recording of human cardiac action potentials on market-available multie...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/10993/41626
Intracellular recording of human cardiac action potentials on market-available multielectrode array platforms
English
Melle, Giovanni [Istituto Italiano di Tecnologia]
Bruno, Giulia []
Maccaferri, Nicolò mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit > ; Istituto Italiano di Tecnologia]
Iachetta, Giuseppina []
Colistra, Nicolò []
Barbaglia, Andrea []
Dipalo, Michele []
De Angelis, Francesco []
In press
Frontiers in Bioengineering and Biotechnology
Frontiers Research Foundation
Yes (verified by ORBilu)
International
2296-4185
Lausanne
Switzerland
[en] bioelectronics ; meta-electrodes ; intracellular recordings ; multi-electrode arrays ; optoacoustic poration ; pharmacology
[en] High quality attenuated intracellular action potentials from large cell networks can be recorded on multi-electrode arrays by means of 3D vertical nanopillars using electrical pulses. However, most of the techniques require complex 3D nanostructures that prevent the straightforward translation into marketable products and the wide adoption in the scientific community. Moreover, 3D nanostructures are often delicate objects that cannot sustain several harsh use/cleaning cycles. On the contrary, laser optoacoustic poration allows the recording of action potentials on planar nanoporous electrodes made of noble metals. However, these constraints of the electrode material and morphology may also hinder the full exploitation of this methodology. Here, we show that optoacoustic poration is also very effective for porating cells on a large family of MEA electrode configurations, including robust electrodes made of nanoporous titanium nitride or disordered fractal-like gold nanostructures. This enables the recording of high quality cardiac action potentials in combination with optoacoustic poration, providing thus attenuated intracellular recordings on various already commercial devices used by a significant part of the research and industrial communities.
http://hdl.handle.net/10993/41626
FP7 ; 616213 - NEURO-PLASMONICS - Neuro-Plasmonics

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Limited access
508133_Dipalo_Manuscript.PDFAuthor preprint5.53 MBRequest a copy

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.