Article (Scientific journals)
Intracellular recording of human cardiac action potentials on market-available multielectrode array platforms
Melle, Giovanni; Bruno, Giulia; Maccaferri, Nicolò et al.
2020In Frontiers in Bioengineering and Biotechnology, 8, p. 66
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Keywords :
bioelectronics; meta-electrodes; intracellular recordings; multi-electrode arrays; optoacoustic poration; pharmacology
Abstract :
[en] High quality attenuated intracellular action potentials from large cell networks can be recorded on multi-electrode arrays by means of 3D vertical nanopillars using electrical pulses. However,most of the techniques require complex 3D nanostructures that prevent the straightforward translation into marketable products and the wide adoption in the scientific community. Moreover, 3D nanostructures are often delicate objects that cannot sustain several harsh use/cleaning cycles. On the contrary, laser optoacoustic poration allows the recording of action potentials on planar nanoporous electrodes made of noble metals. However, these constraints of the electrode material and morphology may also hinder the full exploitation of this methodology. Here, we show that optoacoustic poration is also very effective for porating cells on a large family of MEA electrode configurations, including robust electrodes made of nanoporous titanium nitride or disordered fractal-like gold nanostructures. This enables the recording of high quality cardiac action potentials in combination with optoacoustic poration, providing thus attenuated intracellular recordings on various already commercial devices used by a significant part of the research and industrial communities.
Disciplines :
Biotechnology
Author, co-author :
Melle, Giovanni;  Istituto Italiano di Tecnologia
Bruno, Giulia
Maccaferri, Nicolò ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit ; Istituto Italiano di Tecnologia
Iachetta, Giuseppina
Colistra, Nicolò
Barbaglia, Andrea
Dipalo, Michele
De Angelis, Francesco
External co-authors :
yes
Language :
English
Title :
Intracellular recording of human cardiac action potentials on market-available multielectrode array platforms
Publication date :
2020
Journal title :
Frontiers in Bioengineering and Biotechnology
ISSN :
2296-4185
Publisher :
Frontiers Research Foundation, Lausanne, Switzerland
Volume :
8
Pages :
66
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 616213 - null - Neuro-Plasmonics
Funders :
CE - Commission Européenne [BE]
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