Article (Scientific journals)
The E6 protein from vaccinia virus is required for the formation of immature virions.
Boyd, Olga; Turner, Peter C.; Moyer, Richard W. et al.
2010In Virology, 399 (2), p. 201-11
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Keywords :
Animals; Cell Line; Chlorocebus aethiops; DNA, Viral/biosynthesis/genetics; Isopropyl Thiogalactoside; Mutation; Vaccinia virus/genetics/metabolism/physiology; Viral Core Proteins/genetics/metabolism; Viral Plaque Assay; Virion/physiology; Virus Replication
Abstract :
[en] An IPTG-inducible mutant in the E6R gene of vaccinia virus was used to study the role of the E6 virion core protein in viral replication. In the absence of the inducer, the mutant exhibited a normal pattern DNA replication, concatemer resolution and late gene expression, but it showed an inhibition of virion structural protein processing it failed to produce infectious particles. Electron microscopic analysis showed that in the absence of IPTG viral morphogenesis was arrested before IV formation: crescents, aberrant or empty IV-like structures, and large aggregated virosomes were observed throughout the cytoplasm. The addition of IPTG to release a 12-h block showed that virus infectious particles could be formed in the absence of de novo DNA synthesis. Our observations show that in the absence of E6 the association of viroplasm with viral membrane crescents is impaired.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Boyd, Olga ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Turner, Peter C.
Moyer, Richard W.
Condit, Richard C.
Moussatche, Nissin
External co-authors :
yes
Language :
English
Title :
The E6 protein from vaccinia virus is required for the formation of immature virions.
Publication date :
2010
Journal title :
Virology
ISSN :
1096-0341
Publisher :
Elsevier, Atlanta, United States - California
Volume :
399
Issue :
2
Pages :
201-11
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Published by Elsevier Inc.
Available on ORBilu :
since 06 January 2020

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