Article (Scientific journals)
Live Intracellular Biorthogonal Imaging by Surface Enhanced Raman Spectroscopy using Alkyne-Silver Nanoparticles Clusters
Ardini, Matteo; Huang, Jian-An; Sanchez, Carlos S. et al.
2018In Scientific Reports, 8, p. 12652
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Keywords :
Assay systems; Nanoparticles
Abstract :
[en] Live intracellular imaging is a valuable tool in modern diagnostics and pharmacology. Surface Enhanced Raman Spectroscopy (SERS) stands out as a non-destructive and multiplexed technique, but intracellular SERS imaging still suffers from interfering background from endogenous components. Here we show the assembly of small colloidal SERS probes with Raman signal in the cell-silent window of 1800–2900 cm−1 for biorthogonal intracellular SERS imaging of dopamine that was undistinguishable from the endogenous cell background. By linking colloidal silver nanoparticles with alkyne-dopamine adducts, clusters are formed by 2–6 nanoparticles spaced by tight interparticle gaps that exhibited high electric field enhancement and strong SERS signals of alkyne and dopamines. Due to the cell-silent signals of the alkyne, intracellular in-vitro Raman imaging shows that the dopamines on the internalized clusters remain distinguishable across the cytoplasm with good spatial resolution. Our method can be a general-purpose method for real-time imaging of biomolecules, such as proteins, peptides, DNA and drugs.
Disciplines :
Biotechnology
Author, co-author :
Ardini, Matteo
Huang, Jian-An
Sanchez, Carlos S.
Mousavi, Mansoureh Z.
Caprettini, Valeria
Maccaferri, Nicolò ;  Istituto Italiano di Tecnologia
Melle, Giovanni
Bruno, Giulia
Pasquale, Lea
Garoli, Denis
De Angelis, Francesco
External co-authors :
yes
Language :
English
Title :
Live Intracellular Biorthogonal Imaging by Surface Enhanced Raman Spectroscopy using Alkyne-Silver Nanoparticles Clusters
Publication date :
2018
Journal title :
Scientific Reports
ISSN :
2045-2322
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
8
Pages :
12652
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 616213 - null - Neuro-Plasmonics
Funders :
CE - Commission Européenne [BE]
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