Article (Scientific journals)
Enhanced Raman Investigation of Cell Membrane and Intracellular Compounds by 3D Plasmonic Nanoelectrode Arrays
Caprettini, Valeria; Huang, Jian-An; Moia, Fabio et al.
2018In Advanced Science, 5 (12), p. 1800560
Peer Reviewed verified by ORBi
 

Files


Full Text
Caprettini_et_al-2018-Advanced_Science.pdf
Publisher postprint (3.85 MB)
Download

All documents in ORBilu are protected by a user license.

Send to



Details



Keywords :
Raman spectroscopy; electroporation; intracellular spectroscopy; microelectrode arrays; nanopillars; permeabilization
Abstract :
[en] 3D nanostructures are widely exploited in cell cultures for many purposes such as controlled drug delivery, transfection, intracellular sampling, and electrical recording. However, little is known about the interaction of the cells with these substrates, and even less about the effects of electroporation on the cellular membrane and the nuclear envelope. This work exploits 3D plasmonic nanoelectrodes to study, by surface-enhanced Raman scattering (SERS), the cell membrane dynamics on the nanostructured substrate before, during, and after electroporation. In vitro cultured cells tightly adhere on 3D plasmonic nanoelectrodes precisely in the plasmonic hot spots, making this kind of investigation possible. After electroporation, the cell membrane dynamics are studied by recording the Raman time traces of biomolecules in contact or next to the 3D plasmonic nanoelectrode. During this process, the 3D plasmonic nanoelectrodes are intracellularly coupled, thus enabling the monitoring of different molecular species, including lipids, proteins, and nucleic acids. Scanning electron microscopy cross-section analysis evidences the possibility of nuclear membrane poration compatible with the reported Raman spectra. These findings may open a new route toward controlled intracellular sampling and intranuclear delivery of genic materials. They also show the possibility of nuclear envelope disruption which may lead to negative side effects.
Disciplines :
Biotechnology
Author, co-author :
Caprettini, Valeria
Huang, Jian-An
Moia, Fabio
Jacassi, Andrea
Gonano, Carlo Andrea
Maccaferri, Nicolò ;  Istituto Italiano di Tecnologia
Capozza, Rosario
Dipalo, Michele
De Angelis, Francesco
External co-authors :
yes
Language :
English
Title :
Enhanced Raman Investigation of Cell Membrane and Intracellular Compounds by 3D Plasmonic Nanoelectrode Arrays
Publication date :
2018
Journal title :
Advanced Science
ISSN :
2198-3844
Publisher :
Wiley, Germany
Volume :
5
Issue :
12
Pages :
1800560
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 616213 - null - Neuro-Plasmonics
Funders :
CE - Commission Européenne [BE]
Available on ORBilu :
since 22 February 2019

Statistics


Number of views
55 (6 by Unilu)
Number of downloads
88 (0 by Unilu)

Scopus citations®
 
47
Scopus citations®
without self-citations
40
OpenCitations
 
40
WoS citations
 
42

Bibliography


Similar publications



Contact ORBilu