Article (Scientific journals)
Functionally Convergent B Cell Receptor Sequences in Transgenic Rats Expressing a Human B Cell Repertoire in Response to Tetanus Toxoid and Measles Antigens.
Burckert, Jean-Philippe; Dubois, Axel R. S. X.; Faison, William J. et al.
2017In Frontiers in Immunology, 8, p. 1834
Peer Reviewed verified by ORBi
 

Files


Full Text
fimmu-08-01834.pdf
Publisher postprint (4.3 MB)
Download

All documents in ORBilu are protected by a user license.

Send to



Details



Keywords :
AIRR-seq; B cell repertoire; DESeq2; next-generation sequencing; public CDR3s; repertoire convergence; transgenic rats
Abstract :
[en] The identification and tracking of antigen-specific immunoglobulin (Ig) sequences within total Ig repertoires is central to high-throughput sequencing (HTS) studies of infections or vaccinations. In this context, public Ig sequences shared by different individuals exposed to the same antigen could be valuable markers for tracing back infections, measuring vaccine immunogenicity, and perhaps ultimately allow the reconstruction of the immunological history of an individual. Here, we immunized groups of transgenic rats expressing human Ig against tetanus toxoid (TT), Modified Vaccinia virus Ankara (MVA), measles virus hemagglutinin and fusion proteins expressed on MVA, and the environmental carcinogen benzo[a]pyrene, coupled to TT. We showed that these antigens impose a selective pressure causing the Ig heavy chain (IgH) repertoires of the rats to converge toward the expression of antibodies with highly similar IgH CDR3 amino acid sequences. We present a computational approach, similar to differential gene expression analysis, that selects for clusters of CDR3s with 80% similarity, significantly overrepresented within the different groups of immunized rats. These IgH clusters represent antigen-induced IgH signatures exhibiting stereotypic amino acid patterns including previously described TT- and measles-specific IgH sequences. Our data suggest that with the presented methodology, transgenic Ig rats can be utilized as a model to identify antigen-induced, human IgH signatures to a variety of different antigens.
Disciplines :
Genetics & genetic processes
Author, co-author :
Burckert, Jean-Philippe
Dubois, Axel R. S. X.
Faison, William J.
Farinelle, Sophie
Charpentier, Emilie
Sinner, Regina
WIENECKE-BALDACCHINO, Anke ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Muller, Claude P.
External co-authors :
no
Language :
English
Title :
Functionally Convergent B Cell Receptor Sequences in Transgenic Rats Expressing a Human B Cell Repertoire in Response to Tetanus Toxoid and Measles Antigens.
Publication date :
22 December 2017
Journal title :
Frontiers in Immunology
eISSN :
1664-3224
Publisher :
Frontiers Research Foundation, Lausanne, Switzerland
Volume :
8
Pages :
1834
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 28 March 2018

Statistics


Number of views
72 (0 by Unilu)
Number of downloads
111 (0 by Unilu)

Scopus citations®
 
14
Scopus citations®
without self-citations
14
OpenCitations
 
12
OpenAlex citations
 
17
WoS citations
 
13

Bibliography


Similar publications



Contact ORBilu