Article (Scientific journals)
Revealing new measles virus transmission routes by use of sequence analysis of phosphoprotein and hemagglutinin genes.
Kessler, Julia; Kremer, Jacques R.; Shulga, Sergey V. et al.
2011In Journal of Clinical Microbiology, 49 (2), p. 677-83
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Keywords :
Africa/epidemiology; Cluster Analysis; Disease Outbreaks; Europe/epidemiology; Hemagglutinins, Viral/genetics; Humans; Measles/epidemiology/transmission/virology; Measles virus/classification/genetics/isolation & purification; Molecular Epidemiology; Molecular Sequence Data; Molecular Typing; Nucleoproteins/genetics; Sequence Analysis, DNA; Sequence Homology; Viral Proteins/genetics
Abstract :
[en] With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-HVR sequences. However, these strains showed considerable diversity within a larger sequencing window based on concatenated MV phosphoprotein and hemagglutinin genes (P/H pseudogenes). In Belarus, Germany, Russia, and the Democratic Republic of Congo, the P/H pseudogenes provided insights into chains of transmission, whereas identical NP-HVR provided none. In Russia, for instance, the P/H pseudogene identified temporal clusters rather than geographical clusters, demonstrating the circulation and importation of independent variants rather than large local outbreaks lasting for several years, as suggested by NP-HVR. Thus, by extending the sequencing window for molecular epidemiology, a more refined picture of MV circulation was obtained with more clearly defined links between outbreaks and transmission chains. Our results also suggested that in contrast to the P gene, the H gene acquired fixed substitutions that continued to be found in subsequent outbreaks, possibly with consequences for its antigenicity. Thus, a longer sequencing window has true benefits both for the epidemiological surveillance of measles and for the better monitoring of viral evolution.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kessler, Julia 
Kremer, Jacques R.
Shulga, Sergey V.
Tikhonova, Nina T.
Santibanez, Sabine
Mankertz, Annette
Semeiko, Galina V.
Samoilovich, Elena O.
Tamfum, Jean-Jacques Muyembe
Pukuta, Elisabeth
Muller, Claude P.
Language :
English
Title :
Revealing new measles virus transmission routes by use of sequence analysis of phosphoprotein and hemagglutinin genes.
Publication date :
2011
Journal title :
Journal of Clinical Microbiology
ISSN :
1098-660X
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
49
Issue :
2
Pages :
677-83
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 03 July 2013

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